Use of Clodronate Liposomes to Deplete Phagocytic Immune Cells in Drosophila melanogaster and Aedes aegypti.
Use of Clodronate Liposomes to Deplete Phagocytic Immune Cells in Drosophila melanogaster and Aedes aegypti.
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DOI：
10.3389/fcell.2021.627976
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发表时间：
2021
影响因子：
5.5
通讯作者：
Smith RC
中科院分区：
文献类型：
--
作者：
Ramesh Kumar J;Smith JP;Kwon H;Smith RC
The innate immune system is the primary defense response to limit invading pathogens for all invertebrate species. In insects, immune cells are central to both cellular and humoral immune responses, however few genetic resources exist beyond Drosophila to study immune cell function. Therefore, the development of innovative tools that can be widely applied to a variety of insect systems is of importance to advance the study of insect immunity. Here, we have adapted the use of clodronate liposomes (CLD) to deplete phagocytic immune cells in the vinegar fly, Drosophila melanogaster, and the yellow fever mosquito, Aedes aegypti. Through microscopy and molecular techniques, we validate the depletion of phagocytic cell populations in both insect species and demonstrate the integral role of phagocytes in combating bacterial pathogens. Together, these data demonstrate the wide utility of CLD in insect systems to advance the study of phagocyte function in insect innate immunity. Overview of phagocyte depletion experiments using clodronate liposomes to in Drosophila melanogaster or Aedes aegypti. Created with BioRender.com.
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DOI：
10.1534/g3.117.300452
发表时间：
2018-03-02
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Gyoergy A;Roblek M;Ratheesh A;Valoskova K;Belyaeva V;Wachner S;Matsubayashi Y;Sánchez-Sánchez BJ;Stramer B;Siekhaus DE
通讯作者：
Siekhaus DE
影响因子：
5.2
作者：
Browne N;Heelan M;Kavanagh K
通讯作者：
Kavanagh K
影响因子：
2.9
作者：
Hillyer JF
通讯作者：
Hillyer JF
影响因子：
6.7
作者：
Cockburn IA;Chen YC;Overstreet MG;Lees JR;van Rooijen N;Farber DL;Zavala F
通讯作者：
Zavala F
影响因子：
5.3
作者：
Hillyer, Julian F.;Strand, Michael R.
通讯作者：
Strand, Michael R.