Surface Modification of Melt Extruded Poly(ε-caprolactone) Nanofibers: Toward a New Scalable Biomaterial Scaffold.
Surface Modification of Melt Extruded Poly(ε-caprolactone) Nanofibers: Toward a New Scalable Biomaterial Scaffold.
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DOI：
10.1021/mz500112d
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发表时间：
2014-06-17
影响因子：
7.015
通讯作者：
Pokorski JK
中科院分区：
文献类型：
--
作者：
Kim SE;Wang J;Jordan AM;Korley LT;Baer E;Pokorski JK
描述了熔融挤出的聚合物纳米纤维的光化学改性。生物正交官能团用于装饰仅由商品聚合物制成的纤维，共价连接荧光团和肽，并指导细胞生长。我们的工艺通过使用分层共挤出方法开始，其中聚（ε-己内酯）（PCL）纳米纤维通过挤出生产线内的一系列模头倍增器并入宏观聚（环氧乙烷）（PEO）带中。然后用水洗涤除去PEO层以产生具有受控横截面尺寸的矩形PCL纳米纤维。随后可以使用光化学对纤维进行改性，以产生用于在其表面上进行铜催化的叠氮化物-炔环加成（CuAAC）反应的“可点击”手柄。我们已经附加了荧光团，当使用配体加速的CuAAC反应条件时，其表现出密集的表面覆盖。此外，将RGD肽基序偶联至纤维的表面。随后的基于细胞的研究表明，RGD肽在表面上是生物可及的，导致与PCL对照表面相比增加的细胞粘附和扩散。这种功能化共挤出纤维具有模块化和可扩展性的优点，为生物材料的制造开辟了一条潜在的新途径。
A photochemical modification of melt-extruded polymeric nanofibers is described. A bioorthogonal functional group is used to decorate fibers made exclusively from commodity polymers, covalently attach fluorophores and peptides, and direct cell growth. Our process begins by using a layered coextrusion method, where poly(ε-caprolactone) (PCL) nanofibers are incorporated within a macroscopic poly(ethylene oxide) (PEO) tape through a series of die multipliers within the extrusion line. The PEO layer is then removed with a water wash to yield rectangular PCL nanofibers with controlled cross-sectional dimensions. The fibers can be subsequently modified using photochemistry to yield a “clickable” handle for performing the copper-catalyzed azide–alkyne cycloaddition (CuAAC) reaction on their surface. We have attached fluorophores, which exhibit dense surface coverage when using ligand-accelerated CuAAC reaction conditions. In addition, an RGD peptide motif was coupled to the surface of the fibers. Subsequent cell-based studies have shown that the RGD peptide is biologically accessible at the surface, leading to increased cellular adhesion and spreading versus PCL control surfaces. This functionalized coextruded fiber has the advantages of modularity and scalability, opening a potentially new avenue for biomaterials fabrication.
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影响因子：
3.9
作者：
Causa, Filippo;Battista, Edmond;Netti, Paolo A.
通讯作者：
Netti, Paolo A.
影响因子：
14
作者：
Callahan, Laura A. Smith;Xie, Sibai;Becker, Matthew L.
通讯作者：
Becker, Matthew L.
影响因子：
3.2
作者：
Pokorski, Jonathan K.;Hovlid, Marisa L.;Finn, M. G.
通讯作者：
Finn, M. G.
影响因子：
6.2
作者：
Dongargaonkar, Alpana A.;Bowlin, Gary L.;Yang, Hu
通讯作者：
Yang, Hu
影响因子：
15
作者：
Prucker, O;Naumann, CA;Frank, CW
通讯作者：
Frank, CW