Electrospun blends of gelatin and gelatin-dendrimer conjugates as a wound-dressing and drug-delivery platform.
Electrospun blends of gelatin and gelatin-dendrimer conjugates as a wound-dressing and drug-delivery platform.
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DOI：
10.1021/bm401143p
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发表时间：
2013-11-11
影响因子：
6.2
通讯作者：
Yang, Hu
中科院分区：
文献类型：
--
作者：
Dongargaonkar, Alpana A.;Bowlin, Gary L.;Yang, Hu
在这项工作中，我们报告了一种新的电纺明胶和明胶树枝状聚合物共轭物的混合物的基础上构建。通过EDC/NHS化学将高度支化的星形聚酰胺胺（PAMAM）树枝状聚合物G3.5共价缀合至明胶。将明胶和明胶-树枝状聚合物缀合物与各种负载水平的银乙酸盐（0、0.83、1.65和3.30%w/w）混合的共混物成功地电纺成明胶构建体（NC）。用光反应性聚乙二醇二丙烯酸酯（Mn=575 gmol-1）（PEG DA 575）将NC进一步转化为半互穿网络（sIPN）。他们的特点是在纤维形态，直径，孔径，渗透性，降解和机械性能。所得的sIPN NC保留了纳米纤维的形貌，具有与对应的NC相似的纤维直径，并获得了改善的结构稳定性。由于多孔结构，sIPN NC也显示出良好的溶胀能力，并且对水溶液具有渗透性。含银的sIPN NC允许持续的银释放，并显示出对两种常见类型的病原体-金黄色葡萄球菌和铜绿假单胞菌的抗微生物活性。通过共价结合将树枝状聚合物并入明胶纳米纤维中不仅扩大了明胶构建物的载药能力，而且为开发多功能静电纺丝敷料材料提供了巨大的灵活性。
In this work, we report a new nanofiber construct based on electrospun blends of gelatin and gelatin-dendrimer conjugates. Highly branched star-shaped polyamidoamine (PAMAM) dendrimer G3.5 was covalently conjugated to gelatin via EDC/NHS chemistry. Blends of gelatin and gelatin-dendrimer conjugates mixed with various loading levels of silver acetate (0, 0.83, 1.65, and 3.30% w/w) were successfully electrospun into nanofiber constructs (NCs). The NCs were further converted into semi-interpenetrating networks (sIPNs) with photoreactive polyethylene glycol diacrylate (Mn=575 gmol-1) (PEG DA575). They were characterized in terms of fiber morphology, diameter, pore size, permeability, degradation, and mechanical properties. The resulting sIPN NCs retained nanofiber morphology, possessed similar fiber diameters to counterpart NCs, and gained improved structural stability. The sIPN NCs also showed good swelling capacity owing to porous structures and were permeable to aqueous solutions. Silvercontaining sIPN NCs allowed sustained silver release and showed antimicrobial activity against two common types of pathogens—Staphylococcus aureus and Pseudomonas aeruginosa. Incorporation of dendrimers into the gelatin nanofibers through covalent conjugation not only expands drug loading capacity of nanofiber constructs but provides tremendous flexibility for developing multifunctional electrospun dressing materials.
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影响因子：
9.7
作者：
Kailasan, Arunvel;Yuan, Quan;Yang, Hu
通讯作者：
Yang, Hu
影响因子：
3.1
作者：
Boland, ED;Matthews, JA;Bowlin, GL
通讯作者：
Bowlin, GL
影响因子：
5.8
作者：
PEPPAS, NA;SAHLIN, JJ
通讯作者：
SAHLIN, JJ
影响因子：
9.7
作者：
Aduba, Donald C., Jr.;Hammer, Jeremy A.;Yuan, Quan;Yeudall, W. Andrew;Bowlin, Gary L.;Yang, Hu
通讯作者：
Yang, Hu
DOI：
10.7547/87507315-92-1-24
发表时间：
2002-01-01
影响因子：
0.7
作者：
Seaman, S
通讯作者：
Seaman, S