Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species.
Solid-state NMR analysis of unlabeled fungal cell walls from Aspergillus and Candida species.
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DOI：
10.1016/j.yjsbx.2022.100070
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发表时间：
2022
影响因子：
2.9
通讯作者：
Wang, Tuo
中科院分区：
文献类型：
--
作者：
Fernando, Liyanage D.;Widanage, Malitha C. Dickwella;Shekar, S. Chandra;Mentink-Vigier, Frederic;Wang, Ping;Wi, Sungsool;Wang, Tuo
关键词：
未标记真菌细胞壁的原子分辨率核磁共振研究策略。烟曲霉分生孢子和菌丝体中含有保守的碳水化合物核心。烟曲霉菌中α-葡聚糖结构功能的确认。保存在白色念珠菌液体和固体培养中的碳水化合物指纹。真菌感染导致免疫功能低下者的高死亡率，已成为对人类健康的重大威胁。由于我们对真菌细胞壁的组装和重塑的描述不完整，致力于开发针对细胞壁多糖的抗真菌药物的努力受到了阻碍。高分辨率固体核磁共振研究极大地改变了我们对烟曲霉和其他多种真菌中多糖的多态结构和细胞壁的纳米尺度组织的理解。然而，这种方法需要对所研究的样品进行13C/15N的浓缩，这严重限制了它的应用。在这里，我们使用动态核极化(DNP)技术来比较用液体和固体介质制备的烟曲霉菌和白色念珠菌的未标记细胞壁材料。对于每一种真菌，我们已经从液体和固体培养物中确定了分生孢子和菌丝体细胞壁的高度保守的碳水化合物核心。利用在不同介质中制备的样品，α-葡聚糖与甲壳素包裹形成机械中心的功能最近被证实，通过聚合物动力学的常规SS核磁共振测量得到了证实。这些及时的努力不仅验证了最近发现的烟曲霉细胞壁在不同形态阶段的结构原理，而且也开辟了将目前的研究扩展到其他真菌材料和细胞系统的可能性，这些材料和细胞系统具有标记的挑战性。
An NMR investigation strategy with atomic resolution for unlabeled fungal cell walls. Conserved carbohydrate core revealed in conidia and mycelia of Aspergillus fumigatus. Confirmation of the structural function of α-glucans in A. fumigatus. Carbohydrate fingerprints preserved in liquid and solid cultures of Candida albicans. Fungal infections cause high mortality in immunocompromised individuals, which has emerged as a significant threat to human health. The efforts devoted to the development of antifungal agents targeting the cell wall polysaccharides have been hindered by our incomplete picture of the assembly and remodeling of fungal cell walls. High-resolution solid-state nuclear magnetic resonance (ss NMR) studies have substantially revised our understanding of the polymorphic structure of polysaccharides and the nanoscale organization of cell walls in Aspergillus fumigatus and multiple other fungi. However, this approach requires 13C/15N-enrichment of the sample being studied, severely restricting its application. Here we employ the dynamic nuclear polarization (DNP) technique to compare the unlabeled cell wall materials of A. fumigatus and C. albicans prepared using both liquid and solid media. For each fungus, we have identified a highly conserved carbohydrate core for the cell walls of conidia and mycelia, and from liquid and solid cultures. Using samples prepared in different media, the recently identified function of α-glucan, which packs with chitin to form the mechanical centers, has been confirmed through conventional ss NMR measurements of polymer dynamics. These timely efforts not only validate the structural principles recently discovered for A. fumigatus cell walls in different morphological stages, but also open up the possibility of extending the current investigation to other fungal materials and cellular systems that are challenging to label.
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影响因子：
--
作者：
通讯作者：
--
DOI：
10.1016/j.tcsw.2020.100046
发表时间：
2020-12
期刊：
Cell surface (Amsterdam, Netherlands)
影响因子：
--
作者：
Ehren HL;Appels FVW;Houben K;Renault MAM;Wösten HAB;Baldus M
通讯作者：
Baldus M
影响因子：
4.8
作者：
Chatterjee, Subhasish;Prados-Rosales, Rafael;Stark, Ruth E.
通讯作者：
Stark, Ruth E.
影响因子：
2.9
作者：
Albert, Brice J.;Gao, Chukun;Barnes, Alexander B.
通讯作者：
Barnes, Alexander B.
影响因子：
3.1
作者：
Ibrahim-Granet, O;Philippe, B;Latgé, JP
通讯作者：
Latgé, JP