Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells.
Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells.
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DOI：
10.1002/jbm.a.32781
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发表时间：
2010-09-15
影响因子：
4.9
通讯作者：
Liao, Jun
中科院分区：
文献类型：
--
作者：
Wang, Bo;Borazjani, Ali;Tahai, Mina;Curry, Amy L. de Jongh;Simionescu, Dan T.;Guan, Jianjun;To, Filip;Elder, Steve H.;Liao, Jun
组织工程化心脏移植是一种很有前途的室壁重建治疗模式。近年来，人们发现无细胞组织支架为组织再细胞化和组织重塑提供了天然的超微结构、力学和成分线索。因此，我们评估了脱细胞猪心肌作为厚心脏补片组织工程支架的潜力。取2 mm厚的心肌切片，用0.1%十二烷基硫酸钠(十二烷基硫酸钠)脱细胞，再接种分化的骨髓单个核细胞。我们发现，经过2.5周的治疗后，可以实现彻底的脱细胞。重新接种的细胞在组织构筑物中渗透和增殖。免疫组织化学染色显示，种植的细胞保持心肌细胞样表型，并可能在靠近血管通道的位置发现内皮化，表明有血管生成的潜力。双轴和单轴力学测试均显示脱细胞心肌支架的力学响应较硬；然而，随着培养时间的延长，组织延伸性和拉伸模量值恢复，正如预期的细胞含量增加所预期的那样。我们设想的临床应用的心脏补片将受益于心肌细胞外基质的自然结构，它具有促进干细胞分化、心脏再生和血管生成的潜力。
Tissue engineered cardiac grafts are a promising therapeutic mode for ventricular wall reconstruction. Recently, it has been found that acellular tissue scaffolds provide natural ultrastructural, mechanical, and compositional cues for recellularization and tissue remodeling. We thus assess the potential of decellularized porcine myocardium as a scaffold for thick cardiac patch tissue engineering. Myocardial sections with 2 mm thickness were decellularized using 0.1% sodium dodecyl sulfate (SDS), and then reseeded with differentiated bone marrow mononuclear cells. We found that thorough decellularization could be achieved after 2.5 weeks treatment. Reseeded cells were found to infiltrate and proliferate in the tissue constructs. Immunohistological staining studies showed that the reseeded cells maintained cardiomyocyte-like phenotype and possible endothelialization was found in locations close to vasculature channels, indicating angiogenesis potential. Both biaxial and uniaxial mechanical testing showed a stiffer mechanical response of the acellular myocardial scaffolds; however, tissue extensibility and tensile modulus were found to recover in the constructs along with the culture time, as expected from increased cellular content. The cardiac patch that we envision for clinical application will benefit from the natural architecture of myocardial extracellular matrix, which has the potential to promote stem cell differentiation, cardiac regeneration, and angiogenesis.
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影响因子：
2.7
作者：
De Celle, T;Cleutjens, JP;Janssen, BJ
通讯作者：
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影响因子：
20.1
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Kajstura, J;Rota, M;Anversa, P
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Borschel, GH;Dennis, RG;Kuzon, WM
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Kuzon, WM
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作者：
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通讯作者：
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影响因子：
64.5
作者：
CHIU, CP;BLAU, HM
通讯作者：
BLAU, HM