Small RNA and degradome sequencing reveals microRNAs and their targets involved in tomato pedicel abscission
Small RNA and degradome sequencing reveals microRNAs and their targets involved in tomato pedicel abscission
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小RNA和降解组测序揭示了与番茄蒂脱落有关的microRNA及其靶标
DOI：
10.1007/s00425-015-2318-0
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发表时间：
2015-05
期刊：
影响因子：
4.3
通讯作者：
Li, Tianlai
中科院分区：
文献类型：
--
作者：
Lv, Shuangshuang;Feng, Chaoyang;Qi, Mingfang;Li, Tianlai
主要结论构建了小RNA和降解组测序文库，鉴定了番茄花梗脱落相关的mirna和靶点，并通过实时荧光定量PCR证实了它们的作用。MicroRNAs （miRNAs）是一种内源性小rna，在植物的转录和转录后水平上都起着至关重要的负调控作用。然而，对番茄花梗脱落过程中mirna及其靶基因的表达谱了解有限。利用小RNA （sRNA）和降解组测序技术，在花梗脱落过程中共确认了56个已知和11个新的靶向223个mRNA基因的候选mirna。基因本体注释和KEGG通路分析表明，这些靶基因在细胞内、膜结合细胞器相关的生物过程以及代谢、植物-病原体相互作用和激素信号通路中显著富集。我们筛选了17对miRNA/target对进行进一步分析，并进行了实时荧光定量PCR来确定其作用。对所选miRNAs的聚类分析表明，miRNAs的表达谱在分离的不同阶段存在差异，并可能受到乙烯处理的影响。在本研究中，mirna和靶标之间的相关性表明，在花梗脱落过程中，mirna介导的靶标相互作用存在复杂的调控网络。此外，乙烯改变mirna及其靶点的表达谱可能是乙烯促进花梗脱落的一个重要原因。我们的研究为番茄花梗脱落过程中mirna的表达和调控谱提供了新的见解。
Main conclusionWe constructed small RNA and degradome sequencing libraries to identify miRNAs and targets involved in tomato pedicel abscission, and confirmed their roles via quantitative real-time PCR.MicroRNAs (miRNAs) are endogenous small RNAs which play crucial negatively regulatory roles at both the transcriptional and post-transcriptional levels in plants; however, limited knowledge is available on the expression profiles of miRNAs and their target genes during tomato pedicel abscission. Taking advantage of small RNA (sRNA) and degradome sequencing technology, a total of 56 known and 11 novel candidate miRNAs targeting 223 mRNA genes were confirmed during pedicel abscission. Gene ontology annotation and KEGG pathway analysis showed that these target genes were significantly enriched in intracellular, membrane-bounded organelle-related biological processes as well as in metabolic, plant–pathogen interaction and hormone signaling pathways. We screened 17 miRNA/target pairs for further analysis and performed quantitative real-time PCR to identify the roles. Cluster analysis of selected miRNAs revealed that the expression profiles of miRNAs varied in different stages of abscission and could be impacted by ethylene treatment. In the present study, the correlations between miRNAs and targets suggested a complex regulatory network of miRNA-mediated target interaction during pedicel abscission. Additionally, the expression profiles of miRNAs and their targets changed by ethylene might be a considerable reason why ethylene promotes pedicel abscission. Our study provides new insights into the expression and regulatory profiles of miRNAs during tomato pedicel abscission.
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影响因子：
6.9
作者：
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