Plasma Circular RNAs hsa_circ_0001953 and hsa_circ_0009024 as Diagnostic Biomarkers for Active Tuberculosis.
Plasma Circular RNAs hsa_circ_0001953 and hsa_circ_0009024 as Diagnostic Biomarkers for Active Tuberculosis.
复制标题
血浆环状 RNA hsa_circ_0001953 和 hsa_circ_0009024 作为活动性结核病的诊断生物标志物
DOI：
10.3389/fmicb.2018.02010
复制
发表时间：
2018
影响因子：
5.2
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Huang Z;Su R;Qing C;Peng Y;Luo Q;Li J
近年来的研究表明，环状RNA（circRNA）可作为疾病诊断的潜在分子标志物，但其在活动性结核病（TB）诊断中的价值尚不清楚。本研究首先对来自3名活动性肺结核患者和3名健康对照的血浆样品中的circRNA变化进行了微阵列筛选。然后，选择候选circRNA用于在定量实时PCR系统上验证。在记录的61个差异表达的circRNA中，TB组中分别有43个和18个上调和下调。验证试验表明，6种circRNA，包括hsa_circ_0009024、hsa_circ_0001953、hsa_circ_0008297、hsa_circ_0003528、hsa_circ_0003524和hsa_circ_0015879，在TB患者中的血浆水平显著升高。hsa_circ_0001953和hsa_circ_0009024的血浆水平与TB严重程度相关。接下来，在由120名TB患者和100名对照个体组成的独立队列中评估hsa_circ_0001953和hsa_circ_0009024。联合使用hsa_circ_0001953和hsa_circ_0009024检测TB获得的受试者工作特征（ROC）曲线下面积为0.915（95%置信区间0.880 - 0.951; P <0.001）。此外，治疗后患者血浆hsa_circ_0001953和hsa_circ_0009024水平显著降低（P <0.001）。目前的研究结果表明，circRNA hsa_circ_0001953和hsa_circ_0009024可能代表用于活动性TB诊断的新的血浆生物标志物。
Recent studies have demonstrated that circular RNAs (circRNAs) could serve as potential molecular markers for disease diagnosis; however, little is known about their diagnostic value in active tuberculosis (TB). This study first performed a microarray screening of circRNA changes in plasma samples from 3 patients with active pulmonary TB and 3 healthy controls. Then, candidate circRNAs were selected for validation on a quantitative real-time PCR system. Of the 61 differentially expressed circRNAs recorded, 43 and 18 were upregulated and downregulated in the TB group, respectively. Validation assays demonstrated that plasma levels of 6 circRNAs, including hsa_circ_0009024, hsa_circ_0001953, hsa_circ_0008297, hsa_circ_0003528, hsa_circ_0003524 and hsa_circ_0015879 were remarkably increased in TB patients. Plasma levels of hsa_circ_0001953 and hsa_circ_0009024 were correlated with TB severity. Next, hsa_circ_0001953 and hsa_circ_0009024 were assessed in an independent cohort consisting of 120 TB patients and 100 control individuals. An area under the receiver operating characteristic (ROC) curve of 0.915 (95% confidence interval 0.880-0.951; P < 0.001) was obtained for detecting TB, with hsa_circ_0001953 and hsa_circ_0009024 used in combination. Additionally, plasma levels of hsa_circ_0001953 and hsa_circ_0009024 were reduced significantly in patients after treatment (P < 0.001). The present findings indicate that the circRNAs hsa_circ_0001953 and hsa_circ_0009024 may represent novel plasma biomarkers for active TB diagnosis.
登录
查看更多内容
人单核细胞来源的巨噬细胞对结核分枝杆菌感染反应中差异表达的环状RNA的鉴定
影响因子：
4.6
作者：
Huang Z;Su R;Deng Z;Xu J;Peng Y;Luo Q;Li J
通讯作者：
Li J
影响因子：
5.2
作者：
Sabir N;Hussain T;Shah SZA;Peramo A;Zhao D;Zhou X
通讯作者：
Zhou X
影响因子：
5
作者：
Li, Haixia;Li, Kaifei;Qiu, Yurong
通讯作者：
Qiu, Yurong
影响因子：
--
作者：
Fan X;Weng X;Zhao Y;Chen W;Gan T;Xu D
通讯作者：
Xu D
影响因子：
4.6
作者：
García-Basteiro AL;Ismail MR;Carrilho C;Ussene E;Castillo P;Chitsungo D;Rodríguez C;Lovane L;Vergara A;López-Varela E;Mandomando I;Lorenzoni C;Ordi J;Menéndez C;Bassat Q;Martínez MJ
通讯作者：
Martínez MJ