The Jatropha curcas KASIII gene alters fatty acid composition of seeds in Arabidopsis thaliana

The Jatropha curcas KASIII gene alters fatty acid composition of seeds in Arabidopsis thaliana
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麻风树 KASIII 基因改变拟南芥种子的脂肪酸组成

DOI:
10.1007/s10535-015-0555-5
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发表时间:
2015-10
期刊:
影响因子:
1.5
通讯作者:
C.C. PENG
C.C. PENG
中科院分区:
生物学4区
文献类型:
--
作者:
W.F. XIAO;J. ZHU;X.Y. CHEN;C.C. PENG

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麻疯树(Jatropha curcas L.)是一种多年生、抗旱、非食用油籽作物。种子油的脂肪酸组成,特别是16-碳与18-碳脂肪酸的比例,直接影响到生物柴油的质量。在植物中,脂肪酸链长度主要由质体脂肪酸合成酶复合物决定,该复合物包括三种β-酮酰基-酰基载体蛋白合成酶(KASs)、KASI、KASII和KASIII。KASIII被认为在脂肪酸合成中起限速作用。本文以拟南芥(Arabidopsis thaliana L.)为模型系统,报道麻疯树JcKASIII基因的功能特征。JcKASIII转录本在所有组织中均有表达,在种子中表达量增加。JcKASIII在拟南芥中过表达导致棕榈酸含量增加,16-碳/ 18碳脂肪酸比例增加。此外,对kasI或kasII敲低拟南芥突变体中jaksiii的功能分析显示,种子油的组成发生了变化。综上所述,这些结果表明,异源JcKASIII可能是脂肪酸组成的主要调节因子之一。
Jatropha curcas L. is a perennial, drought-resistant, and non-food oilseed crop. The fatty acid composition of seed oil, especially the ratio of 16- to 18-carbon fatty acids, has a direct impact on the biodiesel quality. In plants, fatty acid chain lengths are mainly determined by the plastidial fatty acid synthase complex which includes three β-ketoacyl-acyl carrier protein synthases (KASs), KASI, KASII, and KASIII. The KASIII is thought to play a rate-limiting role in fatty acid synthesis. Here, we report the functional characterization of a putative JcKASIII gene from Jatropha curcas using Arabidopsis thaliana L. as model system. The transcripts of JcKASIII were detected in all tissues examined and increased in seeds. Overexpression of JcKASIII in Arabidopsis led to an increased content of palmitic acid and a higher ratio of 16- to 18-carbon fatty acids. Moreover, functional analysis of JcAKSIII in kasI or kasII knock down Arabidopsis mutants revealed that the composition of seed oil changed. Taken together, these results suggest that heterologous JcKASIII could function as one of the major regulators of fatty acid composition.
DOI: 10.1007/978-1-4614-4915-7_29
发表时间: 2013
期刊: --
影响因子: --
作者:
Jian Ye;Yan-yun Hong;Jing Qu;Chun Ming Wang
通讯作者: Jian Ye;Yan-yun Hong;Jing Qu;Chun Ming Wang
DOI: 10.1186/1754-6834-7-36
发表时间: 2014-03-08
影响因子: 6.3
作者:
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通讯作者: Chua NH
DOI: 10.1007/s10529-006-0034-3
发表时间: 2006-05
影响因子: 2.7
作者:
L. Tong;S. Peng;Wu-yuan Deng;Dan-Wei Ma;Xu Ying;X. Meng;Chen Fang
通讯作者: L. Tong;S. Peng;Wu-yuan Deng;Dan-Wei Ma;Xu Ying;X. Meng;Chen Fang
DOI: 10.1002/biot.201300231
发表时间: 2013-10
影响因子: 4.7
作者:
Maghuly, Fatemeh;Laimer, Margit
通讯作者: Laimer, Margit
DOI: --
发表时间: 2008-04
影响因子: 2.2
作者:
A. Bamgboye;A. Hansen
通讯作者: A. Bamgboye;A. Hansen