In vitro evidence that bone formation may be coupled to resorption by release of mitogen(s) from resorbing bone.
In vitro evidence that bone formation may be coupled to resorption by release of mitogen(s) from resorbing bone.
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体外证据表明骨形成可能通过从骨吸收中释放丝裂原而与骨吸收相关。
DOI：
10.1016/0026-0495(87)90200-9
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Baylink,DJ
中科院分区：
文献类型：
--
作者：
Farley,JR;Tarbaux,N;Murphy,LA;Masuda,T;Baylink,DJ
骨源性蛋白质已被证明可以刺激骨形成细胞的增殖，并增加体外胚胎骨形成的速率。目前的研究旨在（1）确定鸡胚骨中骨细胞活性有丝分裂原的组织分布，（2）确定鸡胚骨中骨细胞活性有丝分裂原的细胞来源和靶细胞特异性，（3）确定鸡胚骨促有丝分裂活性的释放是否与骨吸收成比例，和（4）比较从不同骨骼来源制备的促有丝分裂活性，关于Mr、化学稳定性和促有丝分裂活性动力学。在骨和软骨提取物中发现了骨细胞活性有丝分裂原，但在肌肉、肝脏、肠或脑提取物中没有发现。（促有丝分裂活性通过在无血清的颅骨细胞培养物中3 [H]-胸苷掺入DNA的增加来确定。）总之，以下三个观察结果表明鸡骨中骨细胞活性丝裂原的成骨细胞来源。首先，在无血清体外培养的8天中，鸡胚胸腺细胞的促分裂原含量增加了4.5倍（P<0.005）。第二，来自颅骨细胞无血清单层培养物的条件培养基（CM）含有骨细胞活性有丝分裂原，但来自皮肤、肝脏和肠细胞平行培养物的CM不含。最后，颅骨细胞CM中骨细胞活性丝裂原的数量与每个细胞中碱性磷酸酶（ALP）活性的数量相关，即成骨细胞分化的指数（r= 0.92，P <0.005）。用放线菌酮处理颅顶细胞引起蛋白质合成和有丝分裂原释放的平行减少（r= 0.99，P <0.01），表明有丝分裂原不在颅顶细胞中储存。从颅骨细胞CM，胫骨CM，和EDTA提取物制备的促有丝分裂活性增加3 [H]-胸苷掺入颅骨细胞培养，但不是在皮肤，肝脏或肠细胞的平行培养。此外，颅骨细胞对这些活性的促有丝分裂反应与ALP活性/细胞成反比（r= 0.97，P <0.001），提示骨祖细胞的相对特异性。在存在骨骼效应物（PTH和Cl 2 MDP）的情况下，胚胎鸡胫骨释放的骨细胞活性丝裂原与骨吸收成正比（r= 0.915，P <0.005），与骨形成成反比（r=-0.80，P <0.05）。当将放线菌酮加入到该系统中时，我们发现有丝分裂原的释放仍然与再吸收相关（r=.996，P < .001），但与蛋白质合成无关（r= .129），这表明CM中的有丝分裂原并不反映新合成的蛋白质。从颅骨细胞CM、胫骨CM和鸡骨EDTA提取物制备的骨细胞活性有丝分裂原不能根据Mr、化学稳定性或有丝分裂原活性动力学相互区分。总之，这些数据表明，骨细胞活性丝裂原由成骨细胞产生，沉积在骨中，与再吸收成比例释放，并且对成骨细胞祖细胞相对特异，并且这些观察结果与成骨细胞可能产生骨体积的延迟旁分泌效应物的假设一致。由于我们还观察到，在颅骨细胞培养物中的ALP活性的量是成正比的有丝分裂原的生产，但成反比的有丝分裂原的反应，这些数据进一步表明，这种有丝分裂活性可能是一个急性旁分泌效应的颅骨细胞增殖在体外。
Bone-derived proteins have been shown to stimulate the proliferation of bone-forming cells and to increase the rate of embryonic bone formation in vitro. The current studies were intended (1) to determine the tissue distribution of bone cell-active mitogen(s) in the embryonic chick, (2) to determine the cellular origin and the target cell specificity of the bone cell-active mitogen(s) in embryonic chick bone, (3) to determine whether the release of mitogenic activity from embryonic chick tibiae was proportional to bone resorption, and (4) to compare mitogenic activities prepared from different skeletal sources, with respect to Mr, chemical stability, and mitogen activity kinetics. A bone cell-active mitogen(s) was identified in extracts of bone and cartilage but not in extracts of muscle, liver, intestine, or brain. (Mitogenic activity was determined as increased incorporation of3[H]-thymidine into DNA in serum-free, calvarial cell cultures.) Together, the following three observations indicate an osteoblastic origin for the bone cell-active mitogen(s) in chick bone. First, the mitogen content of embryonic chick tibiae increased 4.5-fold, during eight days of serum-free in vitro growth (P< .005). Second, conditioned medium (CM) from serum-free monolayer cultures of calvarial cells contained bone cell-active mitogen(s), but CM from parallel cultures of skin, liver, and intestinal cells did not. And, finally, the amount of bone cell-active mitogen(s) in calvarial cell CM was correlated with the amount of alkaline phosphatase (ALP) activity per cell, ie, an index of osteoblastic differentiation (r= .92,P< .005). Treatment of calvarial cells with cycloheximide caused parallel decreases in protein synthesis and mitogen release (r= .99,P< .01), indicating that the mitogen was not stored in calvarial cells. Mitogenic activities prepared from calvarial cell CM, tibial CM, and EDTA-extracts of tibiae increased3[H]-thymidine incorporation in calvarial cell cultures, but not in parallel cultures of skin, liver or intestinal cells. Furthermore, the mitogenic response of calvarial cells to these activities was inversely proportional to ALP activity/cell (r= .97,P< .001), suggesting a relative specificity for osteoprogenitor cells. In the presence of skeletal effectors (PTH and Cl2MDP) the release of bone cell-active mitogen(s) from embryonic chick tibiae was proportional to bone resorption (r= .915,P< .005) and inversely proportional to bone formation (r= −.80,P< .05). When cycloheximide was added to this system we found that mitogen release was still correlated with resorption (r= .996,P< .001), but not with protein synthesis (r= .129), suggesting that the mitogen in CM did not reflect newly synthesized protein. Bone cell-active mitogen(s), prepared from calvarial cell CM, tibial CM, and EDTA-extracts of chick bone, could not be distinguished from each other on the basis of Mr, chemical stability, or mitogen activity kinetics. Together, these data indicate that a bone cell-active mitogen(s) is produced by osteoblasts, deposited in bone, released in proportion to resorption, and relatively specific for osteoblast progenitor cells, and these observations are consistent with the hypothesis that osteoblasts may produce a delayed paracrine effector of bone volume. Since we also observed that the amount of ALP activity in calvarial cell cultures was directly proportional to mitogen production, but inversely proportional to mitogen response, these data further indicate that this mitogenic activity may be an acute paracrine effector of calvarial cell proliferation in vitro.
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用于成骨细胞样细胞和软骨细胞的巨噬细胞衍生生长因子。
DOI：
--
发表时间：
1984
影响因子：
11.1
作者：
L. Rifas;V. Shen;K. Mitchell;W. Peck
通讯作者：
W. Peck
磷脂对提取物、原位和循环中骨骼碱性磷酸酶活性的不同影响。
影响因子：
3.9
作者：
J. Farley;U. Jorch
通讯作者：
U. Jorch
影响因子：
20.3
作者：
E. Canalis
通讯作者：
E. Canalis
DOI：
--
发表时间：
1979
期刊：
The Journal of Periodontology
影响因子：
--
作者：
D. Baylink;Chung Ching Liu
通讯作者：
Chung Ching Liu
骨源性生长因子对兔肋软骨细胞培养物中 DNA、RNA 和蛋白聚糖合成的影响。
DOI：
10.1016/0026-0495(82)90080-4
发表时间：
1982
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Kato,Y;Watanabe,R;Nomura,Y;Tsuji,M;Suzuki,F;Raisz,LG;Canalis,E
通讯作者：
Canalis,E