Conjugated linoleic acid alters growth performance, tissue lipid deposition, and fatty acid composition of darkbarbel catfish (Pelteobagrus vachelli)
Conjugated linoleic acid alters growth performance, tissue lipid deposition, and fatty acid composition of darkbarbel catfish (Pelteobagrus vachelli)
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共轭亚油酸改变黑鲶鱼 (Pelteobagrus vachelli) 的生长性能、组织脂质沉积和脂肪酸组成
DOI：
10.1007/s10695-014-0007-8
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发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
Yanou Yang
中科院分区：
文献类型：
--
作者：
Denghang Yu;Feng Huang;Pengcheng Li;Yanou Yang
脂肪肝综合征是养殖鱼类普遍存在的问题。共轭亚油酸（CLA）作为一种控制哺乳动物脂肪沉积的降脂脂肪酸，近年来受到越来越多的关注。因此，本研究的目的是确定饲料中CLA是否可以降低暗刺鲶鱼（Pelteobagrus vachelli）的组织脂质含量，以及脂质含量的降低是否部分是由于脂质代谢酶活性和脂肪酸谱的改变。本试验旨在研究饲料中添加CLA对暗刺鲶鱼生长、组织脂质沉积和脂肪酸组成的影响。分别饲喂0（对照）、0.5% （cl0.5）、1% （cl1）、2% （cl2）和3% (cl3) CLA水平的5种饲粮。结果表明：2 ~ 3% CLA饲料的特定生长率和饲料转化率显著低于对照组饲料。饲粮CLA水平在1% ~ 3%之间，可降低肝脏脂肪含量和腹腔脂肪含量。2 ~ 3% CLA饲料的肝脏脂蛋白脂肪酶和肝甘油三酯脂肪酶活性显著高于对照组，1 ~ 3% CLA饲料的肝脏胰腺甘油三酯脂肪酶活性显著高于对照组。饲料中CLA掺入肝脏、腹腔脂肪和肌肉脂质，肝脏中CLA掺入比例高于其他组织。肝脏CLA沉积以单不饱和脂肪酸（MUFA）为代价。相比之下，CLA沉积似乎主要是以腹腔脂肪中的多不饱和脂肪酸和n-3多不饱和脂肪酸（PUFA）为代价的，而在肌肉中则是以n-3多不饱和脂肪酸为代价的。本研究结果表明，1%剂量的CLA可以降低暗刺鲶鱼的肝脏脂质含量，而不会对其生长率产生负面影响。这种降脂作用可能部分是由于脂质代谢酶活性的增加和脂肪酸的广泛相互转化。虽然肌肉中的CLA沉积（占总脂肪酸的0.66 - 3.19%）高于天然CLA来源，但当鱼喂食2-3 %的CLA时，鱼肌肉中的EPA （C20:5n-3）似乎同时是消耗性的。
Fatty liver syndrome is a prevalent problem of farmed fish. Conjugated linoleic acid (CLA) has received increased attention recently as a fat-reducing fatty acid to control fat deposition in mammals. Therefore, the aim of the present study was to determine whether dietary CLA can reduce tissue lipid content of darkbarbel catfish (Pelteobagrus vachelli) and whether decreased lipid content is partially due to alterations in lipid metabolism enzyme activities and fatty acid profiles. A 76-day feeding trial was conducted to investigate the effect of dietary CLA on the growth, tissue lipid deposition, and fatty acid composition of darkbarbel catfish. Five diets containing 0 % (control), 0.5 % (CLA0.5), 1 % (CLA1), 2 % (CLA2), and 3 % (CLA3) CLA levels were evaluated. Results showed that fish fed with 2–3 % CLA diets showed a significantly lower specific growth rate and feed conversion efficiency than those fed with the control diet. Dietary CLA decreased the lipid contents in the liver and intraperitoneal fat with the CLA levels from 1 to 3 %. Fish fed with 2–3 % CLA diets showed significantly higher lipoprotein lipase and hepatic triacylglycerol lipase activities in liver than those of fish fed with the control, and fish fed with 1–3 % CLA diets had significantly higher pancreatic triacylglycerol lipase activities in liver than those of fish fed with the control. Dietary CLA was incorporated into liver, intraperitoneal fat, and muscle lipids, with higher percentages observed in liver compared with other tissues. Liver CLA deposition was at the expense of monounsaturated fatty acids (MUFA). In contrast, CLA deposition appeared to be primarily at the expense of MUFA and n-3 polyunsaturated fatty acids (PUFA) in the intraperitoneal fat, whereas in muscle it was at the expense of n-3 PUFA. Our results suggested that CLA at a 1 % dose can reduce liver lipid content without eliciting any negative effect on growth rate in darkbarbel catfish. This lipid-lowering effect could be in part due to an increment in the activity of lipid metabolism enzymes and an extensive interconversion of fatty acids. Although CLA deposition in muscle (0.66–3.19 % of total fatty acids) are higher than presented in natural sources of CLA, EPA (C20:5n-3) in fish muscle appears simultaneously expendable, when the fish fed with 2–3 % CLA.
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DOI：
10.1111/j.1439-0396.1984.tb01411.x
发表时间：
2009-10
影响因子：
2.7
作者：
Sadasivam J. Kaushik;P. Luquet
通讯作者：
Sadasivam J. Kaushik;P. Luquet
影响因子：
4.6
作者：
House, RL;Cassady, JP;Odle, J
通讯作者：
Odle, J
影响因子：
3.6
作者：
Du, ZY;Clouet, P;Liu, YJ
通讯作者：
Liu, YJ
影响因子：
4.2
作者：
Diez, Amalia;Menoyo, David;Bautista, Jose M.
通讯作者：
Bautista, Jose M.
影响因子：
4.5
作者：
Kennedy, Sean R.;Bickerdike, Ralph;Tocher, Douglas R.
通讯作者：
Tocher, Douglas R.