Intermingling of chromosome territories in interphase suggests role in translocations and transcription-dependent associations.
Intermingling of chromosome territories in interphase suggests role in translocations and transcription-dependent associations.
复制标题
DOI：
10.1371/journal.pbio.0040138
复制
发表时间：
2006-05
期刊：
影响因子：
9.8
通讯作者：
Pombo A
中科院分区：
文献类型：
--
作者：
Branco MR;Pombo A
在有丝分裂后，哺乳动物染色体部分地解密集以占据细胞核中的不同区域。目前的模型提出，领土分开的染色质间结构域，丰富的可溶性核机械，只有罕见的染色体间的相互作用，可以通过延长染色质环发生。相反，最近的证据染色质流动性和高频率的染色体易位是一致的显着水平的染色体混合，基因组功能和稳定性的重要后果。在这里，我们使用了一种新的高分辨率原位杂交程序，保留染色质纳米结构，显示染色体领域在人类细胞核中显着混合。人类淋巴细胞中特定染色体对之间的混合程度与同一细胞类型中染色体易位的频率相关，这意味着在混合区域内形成的双链断裂更有可能参与染色体间重排。转录工厂的存在下，在区域的混合和转录损伤的影响，染色体之间的相互作用表明，转录依赖的染色体间协会形状的哺乳动物细胞中的染色体组织。这些发现表明，局部染色质构象和基因转录影响染色体相互作用的程度，并影响其整体性质，直接影响细胞类型特异性基因组稳定性。作者应用了一种新的高分辨率原位杂交方法，该方法保留了染色质纳米结构，并表明染色体区域在人类细胞核中显著混合。
After mitosis, mammalian chromosomes partially decondense to occupy distinct territories in the cell nucleus. Current models propose that territories are separated by an interchromatin domain, rich in soluble nuclear machinery, where only rare interchromosomal interactions can occur via extended chromatin loops. In contrast, recent evidence for chromatin mobility and high frequency of chromosome translocations are consistent with significant levels of chromosome intermingling, with important consequences for genome function and stability. Here we use a novel high-resolution in situ hybridization procedure that preserves chromatin nanostructure to show that chromosome territories intermingle significantly in the nucleus of human cells. The degree of intermingling between specific chromosome pairs in human lymphocytes correlates with the frequency of chromosome translocations in the same cell type, implying that double-strand breaks formed within areas of intermingling are more likely to participate in interchromosomal rearrangements. The presence of transcription factories in regions of intermingling and the effect of transcription impairment on the interactions between chromosomes shows that transcription-dependent interchromosomal associations shape chromosome organization in mammalian cells. These findings suggest that local chromatin conformation and gene transcription influence the extent with which chromosomes interact and affect their overall properties, with direct consequences for cell-type specific genome stability. The authors apply a novel high-resolution in situ hybridization method that preserves chromatin nanostructure and show that chromosome territories intermingle significantly in the nucleus of human cells.
登录
查看更多内容
影响因子：
2.6
作者：
Arsuaga, J;Greulich-Bode, KM;Hlatky, L
通讯作者：
Hlatky, L
转录位点与小麦核中的染色体领土无关。
影响因子：
7.8
作者：
Abranches, R;Beven, A F;Aragon-Alcaide, L;Shaw, P J
通讯作者：
Shaw, P J
影响因子：
4.6
作者：
Laval, JP;Dubrulle, B;Nazarenko, S
通讯作者：
Nazarenko, S
影响因子：
2.6
作者：
Cremer, M;von Hase, J;Cremer, T
通讯作者：
Cremer, T
影响因子：
64.5
作者：
Gilbert, N;Boyle, S;Bickmore, WA
通讯作者：
Bickmore, WA