Different Effects of Albumin and Hydroxyethyl Starch on Low Molecular-Weight Solute Permeation through Sodium Hyaluronic Acid Solution.
Different Effects of Albumin and Hydroxyethyl Starch on Low Molecular-Weight Solute Permeation through Sodium Hyaluronic Acid Solution.
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DOI：
10.3390/polym13040514
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发表时间：
2021-02-09
期刊：
影响因子：
5
通讯作者：
Tatara T
中科院分区：
文献类型：
--
作者：
Tatara T
透明质酸(HA)是一种高相对分子质量的线性多糖，限制了溶质在组织间隙中的运输。白蛋白和羟乙基淀粉(HES)溶液用于纠正手术中血容量的减少，但在炎症条件下可能会渗入间质。考虑到HA的结构可能受到邻近大分子的影响，本研究测试了白蛋白和HES(相对分子质量130,000)对透明质酸钠(NaHA：MW 1.3×106)溶液的溶质渗透是否有不同的影响。为此，评估了合成偶氮染料橙色G在含有白蛋白或HES的NaHA溶液中随时间的渗透情况。在相对于参比溶液(即，不含NaHA的胶体溶液)的每个NaHA溶液中，确定橙色G的相对吸光度达到0.3(T0.3)所需的时间量。白蛋白在0.1%NaHA溶液(3.33±0.69vs.1.16±0.08，p=0.006，n=3)和0.2%NaHA溶液(1.95±0.32vs.0.92±0.27vs.0.92±0.27，p=0.013，n=3)中的相对T0.3值大于HES。这一发现可能有助于选择合适的胶体溶液来控制炎症条件下药物进入癌组织间质的输送。
Hyaluronic acid (HA), a high-molecular-weight linear polysaccharide, restricts solute transport through the interstitial space. Albumin and hydroxyethyl starch (HES) solutions are used to correct the decrease of blood volume during surgery, but may leak into the interstitial space under inflammation conditions. Given the possibility that the structure of HA may be affected by adjacent macromolecules, this study tested whether albumin and HES (Mw 130,000) exert different effects on solute permeation through sodium hyaluronic acid (NaHA: Mw 1.3 × 106) solution. To this end, permeation of Orange G, a synthetic azo dye (Mw 452), into NaHA solutions containing albumin or HES over time was assessed. The amount of time it took for the relative absorbance of Orange G to reach 0.3 (T0.3) was determined in each NaHA solution relative to the reference solution (i.e., colloid solution without NaHA). Relative T0.3 values of albumin were larger than those of HES for 0.1% NaHA solution (3.33 ± 0.69 vs. 1.16 ± 0.08, p = 0.006, n = 3) and 0.2% NaHA solution (1.95 ± 0.32 vs. 0.92 ± 0.27, p = 0.013, n = 3). This finding may help in the selection of an appropriate colloid solution to control drug delivery into the interstitial space of cancer tissue under inflammation conditions.
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影响因子：
6.2
作者：
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