Extracellular Lactate: A Novel Measure of T Cell Proliferation.
Extracellular Lactate: A Novel Measure of T Cell Proliferation.
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细胞外乳酸：T细胞增殖的一种新型量度。
DOI：
10.4049/jimmunol.1700886
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发表时间：
2018-02-01
期刊：
影响因子：
--
通讯作者：
Jones JL
中科院分区：
文献类型：
--
作者：
Grist JT;Jarvis LB;Georgieva Z;Thompson S;Kaur Sandhu H;Burling K;Clarke A;Jackson S;Wills M;Gallagher FA;Jones JL
活化后，T细胞迅速分裂并获得效应子功能。这种能量需求过程取决于T细胞经历从氧化磷酸化到有氧糖酵解的代谢重塑的能力，在此期间葡萄糖转化为乳酸盐并释放到细胞外。在这篇文章中，我们证明，细胞外乳酸可用于动态评估人类T细胞反应在体外。细胞外乳酸水平与T细胞增殖强烈相关，并且测量乳酸与用于确定T细胞应答的传统方法相比是有利的（即，[3H]胸苷掺入和细胞增殖染料的使用）。此外，我们证明了有用的测量乳酸作为读出在传统的抑制试验和高通量肽筛选试验。细胞外乳酸稳定产生超过7天，结果重复进行了几次冻融循环。我们的结论是，使用细胞外乳酸测量可以是一个敏感的，安全的，稳定的，易于实施的研究工具，用于测量T细胞反应和细胞代谢的变化在体外。
Following activation, T cells rapidly divide and acquire effector functions. This energetically demanding process depends upon the ability of T cells to undergo metabolic remodeling from oxidative phosphorylation to aerobic glycolysis, during which glucose is converted into lactate and released extracellularly. In this article, we demonstrate that extracellular lactate can be used to dynamically assess human T cell responses in vitro. Extracellular lactate levels strongly correlated with T cell proliferation, and measuring lactate compared favorably with traditional methods for determining T cell responses (i.e., [3H]thymidine incorporation and the use of cell proliferation dyes). Furthermore, we demonstrate the usefulness of measuring lactate as a read-out in conventional suppression assays and high-throughput peptide-screening assays. Extracellular lactate was stably produced over 7 d, and results were reproducibly performed over several freeze–thaw cycles. We conclude that the use of extracellular lactate measurements can be a sensitive, safe, stable, and easy-to-implement research tool for measuring T cell responses and cellular metabolic changes in vitro.
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