Vascular development and heparin-binding growth factors in the bovine corpus luteum at several stages of the estrous cycle.
Vascular development and heparin-binding growth factors in the bovine corpus luteum at several stages of the estrous cycle.
复制标题
发情周期几个阶段牛黄体中的血管发育和肝素结合生长因子。
作者：
Jing Zheng;D. Redmer;L. Reynolds
免疫定位因子VIII（一个特定的内皮细胞标记物）和肝素结合（成纤维细胞）生长因子（HBGF）-1和HBGF-2，沿着与定量图像分析，被用来评估血管的发展和分布的HBGF在牛黄体（CL）在三个阶段（早期，中期和晚期）的发情周期。黄体血管数量从早期到中期逐渐增加，然后下降到晚期。在早期阶段，大多数微血管存在于组织内折的核心（推测为膜衍生区域），但可以看到许多毛细血管芽侵入实质区域。中期，毛细血管密度大，实质细胞多与一条或多条毛细血管接触。在晚期，相对较少的毛细血管存在于实质领域，而较大的微血管突出整个CL。在整个发情周期中，HBGF-1和HBGF-2主要存在于大、小黄体细胞的细胞质中。在发情周期的各个阶段，HBGF-2的染色强度均大于HBGF-1。相对高水平的HBGF-2，但不是HBGF-1，也存在于结缔组织束。此外，HBGF-1和HBGF-2似乎共同定位于一些黄体细胞。虽然HBGF-1在整个CI中的分布是均匀的，但HBGF-2是不均匀的。对于HBGF-1，染色从早期到中期增加，但从中期到晚期保持不变。相反，HBGF-2染色在中期最大，在早期和晚期相似。这些数据是第一次报告的HBGF-1和HBGF-2免疫定位在整个发情周期的牛黄体组织，并证明，HBGF-2染色遵循类似的模式，黄体血管的发展。
Immunolocalization of factor VIII (a specific endothelial cell marker) and heparin-binding (fibroblast) growth factors (HBGF)-1 and HBGF-2, along with quantitative image analysis, were used to evaluate vascular development and distribution of HBGF in the bovine corpus luteum (CL) at three stages (early, middle, and late) of the estrous cycle. Luteal vascularity increased from the early to the middle stage and then declined to the late stage. During the early stage, most of the microvessels were present in the cores of the tissue infoldings (presumably thecal-derived areas), but numerous capillary sprouts could be seen invading the parenchymal areas. During the middle stage, capillary density was so great that most parenchymal cells were in contact with one or more capillaries. At the late stage, relatively few capillaries were present in the parenchymal areas, whereas larger microvessels were prominent throughout the CL. Throughout the estrous cycle, HBGF-1 and HBGF-2 were present primarily in the cytoplasm of large and small luteal cells. For each stage of the estrous cycle, HBGF-2 staining was greater than that of HBGF-1. Relatively high levels of HBGF-2, but not HBGF-1, were also present in connective tissue tracts. In addition, HBGF-1 and HBGF-2 appeared to co-localize in some luteal cells. Although the distribution of HBGF-1 was homogeneous throughout the CI, that of HBGF-2 was heterogeneous. For HBGF-1, staining increased from the early to the middle stage but remained unchanged from the middle to the late stage. In contrast, HBGF-2 staining was greatest in the middle stage and similar in the early and late stages. These data are the first report of HBGF-1 and HBGF-2 immunolocalization in bovine luteal tissues throughout the estrous cycle, and demonstrate that HBGF-2 staining follows a pattern similar to that of luteal vascular development.
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影响因子：
3.6
作者：
LEI, ZM;CHEGINI, N;RAO, CV
通讯作者：
RAO, CV
DOI：
10.1530/jrf.0.0910001
发表时间：
1991
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
D. Stirling;Michael R. Waterman;Evan R. Simpson
通讯作者：
D. Stirling;Michael R. Waterman;Evan R. Simpson
影响因子：
158.5
作者：
WEIDNER, N;SEMPLE, JP;FOLKMAN, J
通讯作者：
FOLKMAN, J
成纤维细胞生长因子的分子特征：在各种组织中的分布和生物活性。
DOI：
10.1016/b978-0-12-571142-5.50008-2
发表时间：
1986
期刊：
Recent progress in hormone research
影响因子：
--
作者：
Baird,A;Esch,F;Mormède,P;Ueno,N;Ling,N;Böhlen,P;Ying,SY;Wehrenberg,WB;Guillemin,R
通讯作者：
Guillemin,R
影响因子：
4.8
作者：
GOSPODAROWICZ, D;CHENG, J;BOHLEN, P
通讯作者：
BOHLEN, P