A microchip CD4 counting method for HIV monitoring in resource-poor settings.
A microchip CD4 counting method for HIV monitoring in resource-poor settings.
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DOI：
10.1371/journal.pmed.0020182
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发表时间：
2005-07
期刊：
影响因子：
15.8
通讯作者：
McDevitt, JT
中科院分区：
文献类型：
--
作者：
Rodriguez, WR;Christodoulides, N;Floriano, PN;Graham, S;Mohanty, S;Dixon, M;Hsiang, M;Peter, T;Zavahir, S;Thior, I;Romanovicz, D;Bernard, B;Goodey, AP;Walker, BD;McDevitt, JT
发展中国家有超过 3500 万人感染艾滋病毒。全球目前正在进行一项巨大的努力，为至少 300 万感染者提供抗逆转录病毒治疗。尽管药品价格已大幅下降，但治疗 HIV 疾病所必需的实验室检测（例如 CD4 细胞计数）的成本和技术复杂性仍然令人望而却步。迫切需要可在资源匮乏的环境中实施的新的、简单的、负担得起的 CD4 细胞测量方法。在这里，我们描述了一种简单、快速且经济实惠的 CD4 淋巴细胞计数方法原型的开发。无需进一步样品制备的微升血液就可以用荧光抗体染色，捕获在微型流动池内的膜上，并通过显微镜光学器件和为数码相机技术开发的电荷耦合装置类型进行成像。相关的计算机算法将原始数字图像实时转换为绝对 CD4 计数和 CD4 百分比。该原型系统的准确性通过在美国和博茨瓦纳的测试得到了验证，并且在一系列绝对 CD4 计数上与标准流式细胞术 (r = 0.95) 非常一致，并且能够以高灵敏度和特异性区分临床相关的 CD4 计数阈值。将新技术应用于生物医学检测系统（例如本文中描述的系统）的进步有望使艾滋病毒和其他传染病的复杂诊断成为全球现实。一种快速且经济的 CD4 细胞计数方法可能有助于资源匮乏地区的 HIV 管理。
More than 35 million people in developing countries are living with HIV infection. An enormous global effort is now underway to bring antiretroviral treatment to at least 3 million of those infected. While drug prices have dropped considerably, the cost and technical complexity of laboratory tests essential for the management of HIV disease, such as CD4 cell counts, remain prohibitive. New, simple, and affordable methods for measuring CD4 cells that can be implemented in resource-scarce settings are urgently needed. Here we describe the development of a prototype for a simple, rapid, and affordable method for counting CD4 lymphocytes. Microliter volumes of blood without further sample preparation are stained with fluorescent antibodies, captured on a membrane within a miniaturized flow cell and imaged through microscope optics with the type of charge-coupled device developed for digital camera technology. An associated computer algorithm converts the raw digital image into absolute CD4 counts and CD4 percentages in real time. The accuracy of this prototype system was validated through testing in the United States and Botswana, and showed close agreement with standard flow cytometry (r = 0.95) over a range of absolute CD4 counts, and the ability to discriminate clinically relevant CD4 count thresholds with high sensitivity and specificity. Advances in the adaptation of new technologies to biomedical detection systems, such as the one described here, promise to make complex diagnostics for HIV and other infectious diseases a practical global reality. A rapid affordable method for counting CD4 cells may be useful for management of HIV in resource poor settings.
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影响因子：
39.2
作者：
Mellors, JW;Munoz, A;Rinaldo, CR
通讯作者：
Rinaldo, CR
DOI：
10.1002/cyto.10094
发表时间：
2002-04-15
期刊：
CYTOMETRY
影响因子：
--
作者：
Whitby, L;Granger, V;Barnett, D
通讯作者：
Barnett, D
影响因子：
8.8
作者：
Kannangai, R;Prakash, KJ;Sridharan, G
通讯作者：
Sridharan, G
影响因子：
7.4
作者：
Christodoulides, N;Tran, M;McDevitt, JT
通讯作者：
McDevitt, JT
影响因子：
3.3
作者：
Diagbouga, S;Durand, G;Ledru, E
通讯作者：
Ledru, E