Extensive chromosome homoeology among Brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.).
Extensive chromosome homoeology among Brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.).
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DOI：
10.1093/dnares/dsr027
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Nishio T
中科院分区：
文献类型：
--
作者：
Li F;Hasegawa Y;Saito M;Shirasawa S;Fukushima A;Ito T;Fujii H;Kishitani S;Kitashiba H;Nishio T
以表达序列标签(EST)为基础的萝卜分子标记连锁图谱。本研究建立了一种低成本、高效率的单核苷酸多态(SNP)基因分型方法--多重聚合酶链式反应-混合探针斑点杂交技术。将746个来自萝卜EST序列的单核苷酸多态标记划分为9个连锁群，总长度为806.7 cM。通过BLASTN，在拟南芥中发现了726个具有同源基因的标记，并鉴定出72个共线区域，它们在模式物种拟南芥的基因组信息的基础和应用遗传学中具有很大的利用潜力。根据十字花科植物祖先核型中的24个基因组块构建和分析了萝卜的基因组结构，发现24个基因组块中有23个存在于萝卜基因组中，其中一半是三倍体。将大蒜的基因组结构与芥菜科油菜A、B、C基因组以及芥菜科植物Sinapis alba L.的基因组结构进行比较，发现油菜科油菜的基因组结构具有广泛的同源性，这有助于将基因组信息从一个油菜科植物转移到另一个油菜科植物。
A linkage map of expressed sequence tag (EST)-based markers in radish (Raphanus sativus L.) was constructed using a low-cost and high-efficiency single-nucleotide polymorphism (SNP) genotyping method named multiplex polymerase chain reaction–mixed probe dot-blot analysis developed in this study. Seven hundred and forty-six SNP markers derived from EST sequences of R. sativus were assigned to nine linkage groups with a total length of 806.7 cM. By BLASTN, 726 markers were found to have homologous genes in Arabidopsis thaliana, and 72 syntenic regions, which have great potential for utilizing genomic information of the model species A. thaliana in basic and applied genetics of R. sativus, were identified. By construction and analysis of the genome structures of R. sativus based on the 24 genomic blocks within the Brassicaceae ancestral karyotype, 23 of the 24 genomic blocks were detected in the genome of R. sativus, and half of them were found to be triplicated. Comparison of the genome structure of R. sativus with those of the A, B, and C genomes of Brassica species and that of Sinapis alba L. revealed extensive chromosome homoeology among Brassiceae species, which would facilitate transfer of the genomic information from one Brassiceae species to another.
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影响因子：
12.3
作者：
Mun JH;Kwon SJ;Yang TJ;Seol YJ;Jin M;Kim JA;Lim MH;Kim JS;Baek S;Choi BS;Yu HJ;Kim DS;Kim N;Lim KB;Lee SI;Hahn JH;Lim YP;Bancroft I;Park BS
通讯作者：
Park BS
DOI：
10.1073/pnas.0510791103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Lysak, MA;Berr, A;Schubert, I
通讯作者：
Schubert, I
影响因子：
5.4
作者：
Kamei, Akito;Tsuro, Masato;Hirai, Masashi
通讯作者：
Hirai, Masashi
影响因子：
3.1
作者：
Bett, KE;Lydiate, DJ
通讯作者：
Lydiate, DJ
影响因子：
5.4
作者：
Budahn, Holger;Peterka, Herbert;Li, Jinbin
通讯作者：
Li, Jinbin