Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering.
Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering.
复制标题
用于骨组织工程的仿生纳米纤维明胶/磷灰石复合支架。
DOI：
10.1016/j.biomaterials.2008.12.068
复制
发表时间：
2009-04
期刊：
影响因子：
14
通讯作者：
Ma, Peter X.
中科院分区：
文献类型：
--
作者：
Liu, Xiaohua;Smith, Laura A.;Hu, Jiang;Ma, Peter X.
模拟天然细胞外基质(ECM)的某些特征(如纳米级的形貌和生物线索)有利于损伤组织的成功再生。本研究制备了纳米纤维明胶/磷灰石(NF-明胶/磷灰石)复合支架，以模拟自然骨ECM的物理结构和化学组成。发展了一种热诱导相分离(TIPS)技术来制备纳米纤维明胶(NF-明胶)基质。核因子-明胶基质模仿天然胶原纤维，平均纤维直径约150 nm。将TIPS法与致孔剂浸出法相结合，制备了具有良好大孔结构的三维核因子-明胶支架。与孔径和孔隙率相似的Gelfoam®(一种商用明胶泡沫)相比，NF明胶支架的表面积和机械强度要高得多。NF-明胶支架的表面积和压缩模数分别是明胶泡沫支架的700倍和10倍以上。NF-明胶支架具有良好的生物相容性和机械稳定性。为了进一步促进成骨前细胞分化和提高机械强度，通过模拟体液孵育过程，将类骨磷灰石颗粒(<；2μm)引入到核因子-明胶支架表面。SBF处理5d后，NF-明胶/磷灰石支架的机械强度明显高于NF-明胶支架。此外，在NF-明胶/磷灰石复合支架中掺入磷灰石促进了成骨分化。细胞培养4周后，成骨细胞-明胶/磷灰石复合体中BSP和OCN的表达分别是成骨细胞-明胶复合体的5倍和2倍。因此，仿生的核因子-明胶/磷灰石支架是骨组织工程的理想材料。
Mimicking certain features (e.g. nanoscale topography and biological cues) of natural extracellular matrix (ECM) is advantageous for the successful regeneration of damaged tissue. In this study, nanofibrous gelatin/apatite (NF-gelatin/apatite) composite scaffolds have been fabricated to mimic both the physical architecture and chemical composition of natural bone ECM. A thermally induced phase separation (TIPS) technique was developed to prepare nanofibrous gelatin (NF-gelatin) matrix. The NF-gelatin matrix mimicked natural collagen fibers and had an average fiber diameter of about 150 nm. By integrating the TIPS method with porogen leaching, three-dimensional NF-gelatin scaffolds with well-defined macropores were fabricated. In comparison to Gelfoam® (a commercial gelatin foam) with similar pore size and porosity, the NF-gelatin scaffolds exhibited a much higher surface area and mechanical strength. The surface area and compressive modulus of NF-gelatin scaffolds were more than 700 times and 10 times higher than that of Gelfoam®, respectively. The NF-gelatin scaffolds also showed excellent biocompatibility and mechanical stability. To further enhance pre-osteoblast cell differentiation as well as improving mechanical strength, bone-like apatite particles (< 2 μm) were incorporated onto the surface of NF-gelatin scaffolds via a simulated body fluid (SBF) incubation process. The NF-gelatin/apatite scaffolds showed significantly higher mechanical strength than NF-gelatin scaffolds 5 days after SBF treatment. Furthermore, the incorporated apatite in the NF-gelatin/apatite composite scaffold enhanced the ostgeogenic differentiation. The expression of BSP and OCN in the osteoblast-(NF-gelatin/apatite composite) constructs was about 5 times and 2 times higher than in the osteoblast-(NF-gelatin) constructs 4 week after cell culture. The biomimetic NF-gelatin/apatite scaffolds are, therefore, excellent for bone tissue engineering.
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影响因子：
14
作者：
KLEIN, CPAT;PATKA, P;DEGROOT, K
通讯作者：
DEGROOT, K
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
影响因子：
1.9
作者：
FINN, MD;SCHOW, SR;SCHNEIDERMAN, ED
通讯作者：
SCHNEIDERMAN, ED
影响因子：
0.9
作者：
Papay, FA;Morales, L;Zins, J
通讯作者：
Zins, J
DOI：
10.1083/jcb.54.3.626
发表时间：
1972-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Elsdale T;Bard J
通讯作者：
Bard J