Genetic diversity of Calycanthaceae accessions estimated using AFLP markers
Genetic diversity of Calycanthaceae accessions estimated using AFLP markers
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使用 AFLP 标记评估蜡梅科种质的遗传多样性
DOI：
10.1016/j.scienta.2006.12.033
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发表时间：
2007-04
影响因子：
4.3
通讯作者：
中科院分区：
文献类型：
--
作者：
在蜡梅科中，所有物种都是美丽的花灌木，具有很高的观赏价值。尤其是蜡梅，是中国著名的传统香花植物。它已经被广泛种植了一千多年，也被当作盆景和切花来欣赏。本研究采用银染扩增片段长度多态性(AFLP)技术对83份蜡梅科种质资源的亲缘关系进行了分析，其中包括76个蜡梅品种和7份其他品种的遗传关系。8对引物组合共扩增出387条多态性条带，平均每份48.4条。根据Jaccard相似系数和基于UPGMA构建的聚类分析结果表明：(1)浙江蜡梅与其他物种的分化程度较高，与柳叶蜡梅的亲缘关系最近，这可能支持浙江蜡梅是一个独立的物种，而不是毛蜡梅的异名。此外，雷氏蜡梅与蜡梅的亲缘关系远高于其他物种。(2)早熟禾不同基因型间存在广泛的遗传分化。Jaccard相似系数在0.70~0.96之间，根据UPGMA系统聚类图将76份材料划分为7个主要类群。来自两个不同地区的基因型分别聚在一起，表明两个地方栽培群体之间存在遗传差异。但聚类与花形态性状的匹配不是很好，可能证实了早熟禾的花表型性状(花的类型和大小、中花被片颜色和中花被片形状)是受多基因控制的数量性状，而不是质量性状。此外，AFLP标记可用于评价蜡梅科植物间的亲缘关系和蜡梅科品种间的遗传变异，可作为进一步鉴定和分类种质的有效指纹技术。
In Calycanthaceae, all species are attractive flowering shrubs bearing high ornamental value. Especially, Chimonanthus praecox is a famous traditional fragrant flower plant in China. It has been extensively cultivated for over one thousand years and also appreciated as potted landscapes and cut flowers. In this project, silver-stained amplified fragment length polymorphism (AFLP) was applied to detect the genetic relationships among 83 Calycanthaceae accessions, including 76 genotypes from C. praecox and 7 accessions from the other species. Eight primer combinations yielded a total of 387 polymorphic bands, with an average of 48.4 per assay. The results obtained with the analysis of genetic similarities among accessions according to Jaccard's similarity coefficient and the clusters constructed based on the UPGMA indicated that: (1) Chimonanthus zhejiangensis was highly differentiated from the other species and detected the closest relationship with C. salicifolius, which possibly supported that C. zhejiangensis was a distinct species rather than the synonym of C. nitens. Besides, C. praecox was much more closely related with Chimonanthus campanulatus than the other species. (2) Extensive genetic differentiation existed among C. praecox genotypes. The Jaccard's similarity coefficient among the accessions ranged from 0.70 to 0.96, and 76 accessions were divided into seven main groups based on the UPGMA dendrogram. The genotypes from two different areas were clustered, respectively, suggesting genetic divergence between the two local cultivated populations. However, the clusters did not match well with the floral morphological characters, perhaps conforming that the floral phenotypic characters (flower type and size, medium and inner tepal color, and medium tepal shape) of C. praecox were quantitative traits controlled by multi-genes rather than qualitative traits. In addition, the results also demonstrated AFLP markers were useful for evaluating the genetic relationship among species in Calycanthaceae and the genetic variation of C. praecox genotypes, and may serve as efficient fingerprinting technique for further identification and classification of germplasms.
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影响因子：
1.9
作者：
Goremykin, V;Hirsch-Ernst, KI;Hellwig, FH
通讯作者：
Hellwig, FH
影响因子：
3.7
作者：
Chang Roh-Hwei;Ding Chen-Sen
通讯作者：
Chang Roh-Hwei;Ding Chen-Sen
DOI：
10.1515/znc-2004-9-1005
发表时间：
2004-10
期刊：
Zeitschrift für Naturforschung C
影响因子：
--
作者：
Chunhui Deng;Guoxin Song;Yaoming Hu
通讯作者：
Chunhui Deng;Guoxin Song;Yaoming Hu
影响因子：
4.2
作者：
J. Loh;R. Kiew;Andrea Kee;L. Gan;Y. Gan
通讯作者：
J. Loh;R. Kiew;Andrea Kee;L. Gan;Y. Gan
DOI：
--
发表时间：
1958-01
期刊：
University of Kansas science bulletin
影响因子：
--
作者：
R. Sokal;C. Michener
通讯作者：
R. Sokal;C. Michener