Differential expression of fibroblast growth factor‐2 and fibroblast growth factor receptor 1 in a scarring and nonscarring model of CNS injury in the rat
Differential expression of fibroblast growth factor‐2 and fibroblast growth factor receptor 1 in a scarring and nonscarring model of CNS injury in the rat
复制标题
大鼠中枢神经系统损伤疤痕和非疤痕模型中成纤维细胞生长因子-2和成纤维细胞生长因子受体1的差异表达
DOI：
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发表时间：
2001
影响因子：
3.4
通讯作者：
A. Logan
中科院分区：
文献类型：
--
作者：
Conrad S. Smith;M. Berry;Wendy E. Clarke;A. Logan
成人脑损伤导致轴突再生失败和致密纤维胶质瘢痕沉积。促进损伤后轴突再生的治疗策略可能需要抗瘢痕形成策略。在新生儿脑创伤中，疤痕物质不会沉积，轴突通过从头生长或再生穿过损伤部位生长。为了达到治疗目标，重演受伤的成年人的非瘢痕新生儿的反应，瘢痕形成的个体差异如何反映发育障碍的营养反应损伤的理解是必要的。成纤维细胞生长因子-2（FGF-2）的表达受发育调节，并被认为是成年大鼠中枢神经系统创伤反应的调节因子。我们研究了产后5天（dpp）（无瘢痕）和产后16天及成年（瘢痕）大鼠大脑皮质穿透性损伤后FGF-2和成纤维细胞生长因子受体1（FGFR 1）的表达。 原位杂交、免疫组织化学和蛋白质印迹显示，瘢痕大鼠病变周围的反应性星形胶质细胞中FGF-2和FGFR 1 mRNA和蛋白质持续增加，而无瘢痕新生儿的反应明显减弱。这些结果表明，星形胶质细胞FGF-2和FGFR 1表达的变化与成熟中枢神经系统损伤后神经胶质瘢痕形成的成熟模式一致，但在没有进一步实验的情况下推断因果关系还为时过早。
Injury to the adult brain results in abortive axon regeneration and the deposition of a dense fibrous glial scar. Therapeutic strategies to promote postinjury axon regeneration are likely to require antiscarring strategies. In neonatal brain wounds, scar material is not laid down and axons grow across the lesion site, either by de novo growth or regeneration. To achieve the therapeutic goal of recapitulating the nonscarring neonatal response in the injured adult, an understanding of how ontogenic differences in scarring reflect developmental diversities in the trophic response to injury is required. Fibrobast growth factor‐2 (FGF‐2) expression is developmentally regulated and has been implicated as a regulator of the wounding response of the adult rat central nervous system. We have investigated the expression of FGF‐2 and fibroblast growth factor receptor 1 (FGFR1) after penetrating lesions to the cerebral cortex of 5 days post partum (dpp) (nonscarring) and 16 dpp and adult (scarring) rats. In situ hybridization, immunohistochemistry and Western blotting showed robust and sustained increases in FGF‐2 and FGFR1 mRNA and protein in reactive astrocytes around the lesion in scarring rats, a response that was attenuated substantially in the nonscarring neonate. These results demonstrate that changes in astrocyte FGF‐2 and FGFR1 expression are coincident with the establishment of a mature pattern of glial scarring after injury in the maturing central nervous system, but it is premature to infer a causal relationship without further experiments.
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影响因子：
--
作者：
Daniel E. Johnson;L. Williams
通讯作者：
Daniel E. Johnson;L. Williams
成纤维细胞生长因子促进分离的海马神经元的存活并增强神经突的延伸。
DOI：
10.1073/pnas.83.9.3012
发表时间：
1986
影响因子：
11.1
作者：
Walicke,P;Cowan,WM;Ueno,N;Baird,A;Guillemin,R
通讯作者：
Guillemin,R
影响因子：
15.9
作者：
Montero, A;Okada, Y;Hurley, MM
通讯作者：
Hurley, MM
DOI：
10.1016/0165-3806(90)90240-y
发表时间：
1990-03-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
CADAY, CG;KLAGSBRUN, M;FINKLESTEIN, SP
通讯作者：
FINKLESTEIN, SP
影响因子：
3.4
作者：
LOGAN, A;BERRY, M;BAIRD, A
通讯作者：
BAIRD, A