A Testis-Derived Hydrogel as an Efficient Feeder-Free Culture Platform to Promote Mouse Spermatogonial Stem Cell Proliferation and Differentiation
A Testis-Derived Hydrogel as an Efficient Feeder-Free Culture Platform to Promote Mouse Spermatogonial Stem Cell Proliferation and Differentiation
复制标题
睾丸源性水凝胶作为有效的无饲养层培养平台，促进小鼠精原干细胞增殖和分化
DOI：
10.3389/fcell.2020.00250
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发表时间：
2020-05
影响因子：
5.5
通讯作者：
Huang Yadong
中科院分区：
文献类型：
--
作者：
Yang Yan;Lin Qilian;Zhou Chengxing;Li Quan;Li Ziyi;Cao Zhen;Liang Jinlian;Li Hanhao;Mei Jiaxin;Zhang Qihao;Xiang Qi;Xue Wei;Huang Yadong
保存生育能力和辅助生殖医学需要有效的培养系统，使精原干细胞（SSCs）成功增殖和分化。许多SSC培养系统需要在每次传代培养中添加饲养细胞，这是繁琐而低效的。本研究从睾丸组织中制备脱细胞睾丸基质（DTM），保留了睾丸必需的结构蛋白。然后将DTM溶解并诱导形成具有随机定向纤维结构的多孔水凝胶支架，具有良好的细胞相容性。在DTM水凝胶支架上接种的SSCs的活力显著高于在Matrigel或层粘连蛋白上接种的SSCs，细胞内基因表达和DNA印迹模式与天然SSCs相似。此外，DTM促进SSC向圆形精子分化。更重要的是，DTM水凝胶支持SSC增殖和分化，而不需要额外的体细胞。DTM水凝胶支架培养系统为SSCs的培养提供了一种替代和简单的方法，消除了饲养细胞引起的潜在变异性和污染。它可能是生殖医学的一个有价值的工具。
Fertility preservation and assisted reproductive medicine require effective culture systems for the successful proliferation and differentiation of spermatogonial stem cells (SSCs). Many SSC culture systems require the addition of feeder cells at each subculture, which is tedious and inefficient. Here, we prepared decellularized testicular matrix (DTM) from testicular tissue, which preserved essential structural proteins of testis. The DTM was then solubilized and induced to form a porous hydrogel scaffold with randomly oriented fibrillar structures that exhibited good cytocompatibility. The viability of SSCs inoculated onto DTM hydrogel scaffolds was significantly higher than those inoculated on Matrigel or laminin, and intracellular gene expression and DNA imprinting patterns were similar to that of native SSCs. Additionally, DTM promoted SSC differentiation into round spermatids. More importantly, the DTM hydrogel supported SSC proliferation and differentiation without requiring additional somatic cells. The DTM hydrogel scaffold culture system provided an alternative and simple method for culturing SSCs that eliminates potential variability and contamination caused by feeder cells. It might be a valuable tool for reproductive medicine.
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影响因子：
3.8
作者：
Tibbitt, Mark W.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.
影响因子：
16.6
作者：
Pati F;Jang J;Ha DH;Won Kim S;Rhie JW;Shim JH;Kim DH;Cho DW
通讯作者：
Cho DW
影响因子：
14
作者：
Romero-López M;Trinh AL;Sobrino A;Hatch MM;Keating MT;Fimbres C;Lewis DE;Gershon PD;Botvinick EL;Digman M;Lowengrub JS;Hughes CC
通讯作者：
Hughes CC
影响因子：
3.8
作者：
Choi NY;Park YS;Ryu JS;Lee HJ;Araúzo-Bravo MJ;Ko K;Han DW;Schöler HR;Ko K
通讯作者：
Ko K
用脱细胞外基质支架控制干细胞行为。
DOI：
10.1016/j.cossms.2016.02.001
发表时间：
2016-08
影响因子：
11
作者：
Agmon G;Christman KL
通讯作者：
Christman KL