Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen.
Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen.
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DOI：
10.1007/s10029-021-02396-7
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Klinge U
中科院分区：
文献类型：
--
作者：
Dievernich A;Achenbach P;Davies L;Klinge U
聚丙烯（PP）补片广泛用于增强组织。对植入物的异物反应（FBR）主要由先天免疫细胞，尤其是巨噬细胞。然而，也经常观察到相当数量的适应性免疫细胞，即T细胞，它们似乎在长期宿主反应中起着至关重要的作用。本研究调查了7个人PP补片的FBR，这些补片在中位时间1年后从腹部取出以防止复发。使用免疫荧光显微镜检查FBR中的各种先天性（CD 11 B+骨髓、CD 68+巨噬细胞、CD 56 + NK）和适应性免疫细胞（CD 3 + T、CD 4 + T辅助细胞、CD 8+细胞毒性、FoxP 3 + T调节细胞、CD 20 + B）以及“常规”免疫细胞（定义为表达其特异性免疫细胞标记物但不共表达CD 68的细胞）。辅助性T细胞（19%）和调节性T细胞（25%）以与巨噬细胞相当的比率存在，并向网状纤维显著聚集。对于所有细胞类型，在网状物-组织界面处观察到最低比例的“常规”细胞（< 60%），但在约50-100 µm处显著增加，表明随着与网状物纤维距离的增加刺激减少。先天性和适应性免疫细胞都参与慢性FBR到PP网，其中T细胞和巨噬细胞分别是主要的细胞类型。与先前的数据一致，许多细胞在网状纤维附近呈现“混合”模式。异物肉芽肿内免疫反应的复杂性可以解释为什么专注于特定细胞类型的方法在降低慢性FBR方面并不非常成功。在线版本包含补充材料，可通过10.1007/s10029-021-02396-7获得。
Polypropylene (PP) mesh is widely used to reinforce tissues. The foreign body reaction (FBR) to the implant is dominated by innate immune cells, especially macrophages. However, considerable numbers of adaptive immune cells, namely T cells, have also been regularly observed, which appear to play a crucial role in the long-term host response. This study investigated the FBR to seven human PP meshes, which were removed from the abdomen for recurrence after a median of one year. Using immunofluorescence microscopy, the FBR was examined for various innate (CD11b+ myeloid, CD68+ macrophages, CD56+ NK) and adaptive immune cells (CD3+ T, CD4+ T-helper, CD8+ cytotoxic, FoxP3+ T-regulatory, CD20+ B) as well as “conventional” immune cells (defined as cells expressing their specific immune cell marker without co-expressing CD68). T-helper cells (19%) and regulatory T-cells (25%) were present at comparable rates to macrophages, and clustered significantly toward the mesh fibers. For all cell types the lowest proportions of “conventional” cells (< 60%) were observed at the mesh–tissue interface, but increased considerably at about 50–100 µm, indicating reduced stimulation with rising distance to the mesh fibers. Both innate and adaptive immune cells participate in the chronic FBR to PP meshes with T cells and macrophages being the predominant cell types, respectively. In concordance with the previous data, many cells presented a “hybrid” pattern near the mesh fibers. The complexity of the immune reaction seen within the foreign body granuloma may explain why approaches focusing on specific cell types have not been very successful in reducing the chronic FBR. The online version contains supplementary material available at 10.1007/s10029-021-02396-7.
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影响因子：
4
作者：
Krzyszczyk P;Schloss R;Palmer A;Berthiaume F
通讯作者：
Berthiaume F
DOI：
10.1007/s10029-020-02315-2
发表时间：
2020-12
期刊：
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影响因子：
--
作者：
Dievernich A;Achenbach P;Davies L;Klinge U
通讯作者：
Klinge U
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作者：
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作者：
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1.8
作者：
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