Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation.
Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation.
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DOI：
10.1016/j.virol.2007.03.018
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发表时间：
2007-06-20
期刊：
影响因子：
3.7
通讯作者：
Saif LJ
中科院分区：
文献类型：
--
作者：
Zhang X;Hasoksuz M;Spiro D;Halpin R;Wang S;Vlasova A;Janies D;Jones LR;Ghedin E;Saif LJ
张晓明，张晓明，张晓明，等。直接从饲养小牛鼻（呼吸道）和直肠（肠道）样本中检测到的野生型牛冠状病毒（BCoV） AH65和AH187的遗传多样性[j]。呼吸道和肠源性牛冠状病毒刺突糖蛋白S1亚基的分子分析。病毒学报，84(1-2),101-109。进行了分析。全基因组序列分析显示，AH65和AH187的呼吸道和肠道菌株在整个基因组中分别存在123和149个核苷酸（nt）的差异，表明存在宿主内BCoV准种。此外，在一些BCoV-R和BCoV-E菌株的基因组中发现了大量的序列模糊性，提示分离内准种。AH65呼吸BCoV （AH65- r -TC）和肠BCoV （AH65- e -TC）分别在人直肠肿瘤细胞（HRT-18）中经过14代和15代和1个斑块纯化后，对组织培养（TC）传代的对应体进行测序。与亲本野生型菌株相比，AH65-E-TC分离株的组织培养传代产生104 nt变化，而AH65-R-TC分离株仅产生8 nt变化。特别值得注意的是，AH65-E-TC分离株的大部分核苷酸变化发生在与来自同一动物的AH65-R菌株发生突变的相同位置。这些数据表明，BCoV通过准种发育进化，肠道BCoV分离株更容易发生遗传变化，并可能在组织培养传代后突变为类似呼吸道BCoV菌株。
The genetic diversity of 2 pairs (AH65 and AH187) of wild type bovine coronaviruses (BCoV) sequenced directly from nasal (respiratory) and rectal (enteric) swabs of two feedlot calves with respiratory and enteric symptoms [Hasoksuz, M., Sreevatsan, S., Cho, K.O., Hoet, A.E., Saif, L.J., 2002b. Molecular analysis of the S1 subunit of the spike glycoprotein of respiratory and enteric bovine coronavirus isolates. Virus Res. 84 (1–2), 101–109.]. was analyzed. Sequence analysis of the complete genomes revealed differences at 123 and 149 nucleotides (nt) throughout the entire genome between the respiratory and enteric strains for samples AH65 and AH187, respectively, indicating the presence of intra-host BCoV quasispecies. In addition, significant numbers of sequence ambiguities were found in the genomes of some BCoV-R and BCoV-E strains, suggesting intra-isolate quasispecies. The tissue culture (TC) passaged counterparts of AH65 respiratory BCoV (AH65-R-TC) and enteric BCoV (AH65-E-TC) were also sequenced after 14 and 15 passages and 1 plaque purification in human rectal tumor cells (HRT-18), respectively. Compared to the parental wild type strains, tissue culture passage generated 104 nt changes in the AH65-E-TC isolate but only 8 nt changes in the AH65-R-TC isolate. Particularly noteworthy, the majority of nucleotide changes in the AH65-E-TC isolate occurred at the identical positions as the mutations occurring in the AH65-R strain from the same animal. These data suggest that BCoV evolves through quasispecies development, and that enteric BCoV isolates are more prone to genetic changes and may mutate to resemble respiratory BCoV strains after tissue culture passage.
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影响因子：
2.7
作者：
Hasoksuz M;Lathrop S;Al-dubaib MA;Lewis P;Saif LJ
通讯作者：
Saif LJ
影响因子：
3.8
作者：
Chouljenko, VN;Lin, XQ;Gorbalenya, AE
通讯作者：
Gorbalenya, AE
影响因子：
3.7
作者：
Ballesteros, ML;Sanchez, CM;Enjuanes, L
通讯作者：
Enjuanes, L
影响因子：
3.8
作者：
Jerzak, G;Bernard, KA;Ebel, GD
通讯作者：
Ebel, GD
影响因子：
56.9
作者：
HOLLAND, J;SPINDLER, K;VANDEPOL, S
通讯作者：
VANDEPOL, S