Flat-twisted tubing: novel column design for spiral high-speed counter-current chromatography.
Flat-twisted tubing: novel column design for spiral high-speed counter-current chromatography.
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DOI：
10.1016/j.chroma.2009.05.024
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发表时间：
2009-07-03
期刊：
影响因子：
--
通讯作者：
Ito Y
中科院分区：
文献类型：
--
作者：
Yang Y;Aisa HA;Ito Y
用于高速逆流色谱（HSCCC）的原始螺旋管组件通过一种称为“平扭管”的新管配置得到进一步改进，该管是通过将管（1.6 mm内径）挤出一个狭窄的槽，然后沿其轴线旋转形成约1厘米的扭螺距制成的。这种改变阻断了流动相通过管的层流，使两相通过柱连续混合。采用三种不同极性的两相溶剂体系，分别选取合适的dnp -氨基酸、二肽和蛋白质等样品，在最佳洗脱模式下对该螺旋管组件的性能进行了测试。总的来说，所有这些测试样品的下流动相的分辨率都比上流动相高。在由己烷-乙酸乙酯-甲醇-0.1 m盐酸（1:1:1:1,v/v）组成的最疏水的两相溶剂体系中，采用Rs-a（峰值分辨率基于相同的柱容量进行调整，以进行比较），在流速0.5 ml/min下，以4.40%和73%的固定相保留率分离dnp -氨基酸。在由1-丁醇-乙酸-水（4:1:5,v/v）组成的极性溶剂体系中，双肽样品以4.06的Rs-a溶解，而在45%的固定相保留下，以2.79的Rs-a溶解，流速均为1 ml/min。最后，在由聚乙二醇1000 -二碱性磷酸钾各占12.5% （w/w）水组成的水-水聚合物相体系中，蛋白质样品用Rs-a以2.53的速度溶解，而用交叉压管组件以52%的固定相保留率以1.10的速度溶解，流速均为1 ml/min。这些结果表明，该系统大大提高了配分效率，并在较低的流动相中保留了令人满意的固定相。
The original spiral tube assembly for high-speed counter-current chromatography (HSCCC) is further improved by a new tube configuration called “flat-twisted tubing” which was made by extruding the tube (1.6 mm I.D.) through a narrow slot followed by twisting along its axis forming about 1 cm twisted screw pitch. This modification interrupts the laminar flow of the mobile phase through the tube and continuously mixes the two phases through the column. The performance of this spiral tube assembly was tested by three types of two-phase solvent systems with different polarity each with a set of suitable test samples such as DNP-amino acids, dipeptides and proteins at the optimal elution modes. In general all these test samples yielded higher resolution with the lower mobile phase than the upper mobile phase. In the most hydrophobic two-phase solvent system composed of hexane-ethyl acetate-metanol-0.1M hydrochloric acid (1:1:1:1, v/v), DNP-amino acids were separated with Rs-a (peak resolution based on the same column capacity adjusted for comparison) at 4.40 and 73 % of stationary phase retention at a flow rate of 0.5 ml/min with the lower mobile phase. In the polar solvent system composed of 1-butanol-acetic acid-water (4: 1: 5, v/v), dipeptide samples were resolved with Rs-a at 4.06, compared to 2.79 with the cross-pressed tube assembly at 45 % stationary phase retention, each at a flow rate of 1 ml/min. Finally in the aqueous-aqueous polymer phase systems composed of polyethylene glycol 1000 – dibasic potassium phosphate each 12.5% (w/w) in water, protein samples were resolved with Rs-a at 2.53 compared to 1.10 with the cross-pressed tube assembly at 52 % of stationary phase retention, each at a flow rate of 1 ml/min. These results indicate that the present system substantially improves the partition efficiency with a satisfactory level of stationary phase retention by the lower mobile phase.
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DOI：
10.1080/10826070802019913
发表时间：
2008-01-01
影响因子：
1.3
作者：
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影响因子：
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DOI：
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发表时间：
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DOI：
10.1081/jlc-120021255
发表时间：
2003-01-01
影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
1985-01-01
期刊：
JOURNAL OF LIQUID CHROMATOGRAPHY
影响因子：
--
作者：
CONWAY, WD;ITO, Y
通讯作者：
ITO, Y