The use of fiber-reinforced scaffolds cocultured with Schwann cells and vascular endothelial cells to repair rabbit sciatic nerve defect with vascularization.
The use of fiber-reinforced scaffolds cocultured with Schwann cells and vascular endothelial cells to repair rabbit sciatic nerve defect with vascularization.
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DOI：
10.1155/2013/362918
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发表时间：
2013
影响因子：
--
通讯作者：
Shen Y
中科院分区：
文献类型：
--
作者：
Gao H;You Y;Zhang G;Zhao F;Sha Z;Shen Y
为探讨生物可降解纤维增强三维支架材料修复兔坐骨神经长距离缺损的可行性及早期血管化移植对神经功能恢复的影响，将雪旺细胞与血管内皮细胞在纤维增强三维支架材料中共培养。实验组采用预血管化神经复合体修复坐骨神经缺损，对照组仅采用雪旺细胞培养。术后4、8、16周分别行肌电图检查，观察神经功能恢复情况。光镜和电镜下观察坐骨神经再生和髓鞘形成情况。应用图像分析系统对髓鞘厚度、轴突直径和有髓神经纤维数量进行定量分析。实验组在足部溃疡愈合、神经传导速度、再生神经纤维数量及超微结构恢复方面均优于对照组。预血管化组织工程纤维增强三维支架修复兔坐骨神经缺损可有效促进神经功能恢复。
To explore the feasibility of biodegradable fiber-reinforced 3D scaffolds with satisfactory mechanical properties for the repair of long-distance sciatic nerve defect in rabbits and effects of vascularized graft in early stage on the recovery of neurological function, Schwann cells and vascular endothelial cells were cocultured in the fiber-reinforced 3D scaffolds. Experiment group which used prevascularized nerve complex for the repair of sciatic nerve defect and control group which only cultured with Schwann cells were set. The animals in both groups underwent electromyography to show the status of the neurological function recovery at 4, 8, and 16 weeks after the surgery. Sciatic nerve regeneration and myelination were observed under the light microscope and electron microscope. Myelin sheath thickness, axonal diameter, and number of myelinated nerve fiber were quantitatively analyzed using image analysis system. The recovery of foot ulcer, the velocity of nerve conduction, the number of regenerating nerve fiber, and the recovery of ultrastructure were increased in the experimental group than those in the control group. Prevascularized tissue engineered fiber-reinforced 3D scaffolds for the repair of sciatic nerve defects in rabbits can effectively promote the recovery of neurological function.
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影响因子：
14
作者：
Cao, Lingyan;Wang, Jing;Liu, Changsheng
通讯作者：
Liu, Changsheng
影响因子：
--
作者：
Evans, GRD
通讯作者：
Evans, GRD
影响因子：
4.9
作者：
Koepsell, Laura;Remund, Tyler;Deng, Ying
通讯作者：
Deng, Ying
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影响因子：
14
作者：
Cao, Jiani;Sun, Changkai;Dai, Jianwu
通讯作者：
Dai, Jianwu
骨诱导磷酸钙陶瓷诱导人脂肪干细胞成骨分化
DOI：
10.1002/jbm.b.31773
发表时间：
2011-04-01
影响因子：
3.4
作者：
Li, Xiaoming;Liu, Haifeng;Watari, Fumio
通讯作者：
Watari, Fumio