Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein

Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein
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基因编码的叠氮苯光化学驱动红色荧光蛋白的正向和负向功能调节

DOI:
10.1039/c5ra13552d
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发表时间:
2015
期刊:
影响因子:
3.9
通讯作者:
Reddington S
Reddington S
中科院分区:
化学3区
文献类型:
--
作者:
Reddington S

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苯叠氮光化学性质已被用来调节红色自发荧光蛋白mCherry的功能。利用遗传密码重新编程,苯叠氮化物化学已被引入到小樱桃的功能关键位置,导致在紫外线照射下失活、激活或增强。
The photochemical properties of phenyl azide have been exploited to modulate the function of a red autofluorescent protein, mCherry. Using genetic code reprogramming, phenyl azide chemistry has been introduced at functionally strategic positions in mCherry leading to deactivation, activation or enhancement upon UV irradiation.
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