Prognostic Implication and Oncogenic Role of PNPO in Pan-Cancer.
Prognostic Implication and Oncogenic Role of PNPO in Pan-Cancer.
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PNPO 在泛癌中的预后意义和致癌作用
DOI：
10.3389/fcell.2021.763674
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发表时间：
2021
影响因子：
5.5
通讯作者：
Xu G
中科院分区：
文献类型：
--
作者：
Zhang L;Li X;Zhang J;Xu G
目的：吡哆醇5′-磷酸氧化酶（Pyridoxine 5′-phosphate oxidase， PNPO）是维生素B6代谢的关键酶，影响卵巢癌和乳腺癌的发生。然而，PNPO在其他类型癌症中的作用尚不清楚。方法：采用Cancer Genome Atlas （TCGA）数据库和gentype Tissue-Expression （GTEX）数据库对PNPO的表达进行分析。通过cBioPortal数据库和人体多器官组织阵列分析PNPO基因组改变和人体有机组织中的蛋白表达。PNPO与药敏分析在CellMiner数据库中进行。使用TCGA、ESTIMATE算法和TIMER数据库分析PNPO表达与生存结果、临床特征、DNA错配修复系统（MMR）、微卫星不稳定性（MSI）、肿瘤突变负担（TMB）和免疫相关细胞浸润之间的相关性。应用基因集富集分析（GSEA）研究PNPO在泛癌中的生物学功能。结果：差异分析显示，与正常组织相比，PNPO mRNA在21种肿瘤类型中表达水平上调，这与PNPO蛋白在大多数肿瘤类型中的表达一致。PNPO的异常表达可以预测食管癌（ESCA）、肾透明细胞癌（KIRC）、前列腺癌（PRAD）、卵巢浆液性囊腺癌（OV）、葡萄膜黑色素瘤（UVM）患者的生存结局。此外，PNPO基因组最常见的突变类型被扩增。此外，PNPO的异常表达与不同类型肿瘤的MMR、MSI、TMB和药物敏感性有关。通过ESTIMATE算法和TIMER数据库挖掘，发现PNPO的表达与泛癌组织中各种免疫相关细胞的浸润水平相关。结论：PNPO是一种潜在的分子生物标志物，可用于预测泛癌患者的预后、药物敏感性和免疫反应。
Objective: Pyridoxine 5′-phosphate oxidase (PNPO) is a key enzyme in the metabolism of vitamin B6 and affects the tumorigenesis of ovarian and breast cancers. However, the roles of PNPO in other types of cancer remain unknown. Methods: The expression of PNPO was interpreted by The Cancer Genome Atlas (TCGA) database and Genotype Tissue-Expression (GTEX) database. Analysis of PNPO genomic alterations and protein expression in human organic tissues was analyzed by the cBioPortal database and human multiple organ tissue arrays. PNPO with drug sensitivity analysis was performed from the CellMiner database. The correlations between PNPO expression and survival outcomes, clinical features, DNA mismatch repair system (MMR), microsatellite instability (MSI), tumor mutation burden (TMB), and immune-associated cell infiltration were analyzed using the TCGA, ESTIMATE algorithm, and TIMER databases. Gene Set Enrichment Analysis (GSEA) was applied to elucidate the biological function of PNPO in pan-cancer. Results: The differential analysis showed that the level of PNPO mRNA expression was upregulated in 21 tumor types compared with normal tissues, which was consistent with its protein expression in most cancer types. The abnormal expression of PNPO could predict the survival outcome of patients with esophageal carcinoma (ESCA), kidney renal clear cell carcinoma (KIRC), prostate adenocarcinoma (PRAD), ovarian serous cystadenocarcinoma (OV), and uveal melanoma (UVM). Furthermore, the most frequent mutation type of PNPO genomic was amplified. Moreover, the aberrant PNPO expression was related to MMR, MSI, TMB, and drug sensitivity in various types of cancer. The expression of PNPO was related to the infiltration levels of various immune-associated cells in pan-cancer by ESTIMATE algorithm and TIMER database mining. Conclusion: Our results suggest that PNPO is a potential molecular biomarker for predicting patient prognosis, drug sensitivity, and immunoreaction in pan-cancer.
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影响因子：
5.6
作者：
Mascolo, Elisa;Amoroso, Noemi;Verni, Fiammetta
通讯作者：
Verni, Fiammetta
影响因子：
4.6
作者：
Bonneville R;Krook MA;Kautto EA;Miya J;Wing MR;Chen HZ;Reeser JW;Yu L;Roychowdhury S
通讯作者：
Roychowdhury S
影响因子：
7.1
作者：
MEYDANI, SN;RIBAYAMERCADO, JD;GERSHOFF, SN
通讯作者：
GERSHOFF, SN
DOI：
10.1136/adc.2006.115162
发表时间：
2008-03-01
影响因子：
4.4
作者：
Bagci, S.;Zschocke, J.;Franz, A. R.
通讯作者：
Franz, A. R.
影响因子：
1.6
作者：
Doke, S;Inagaki, N;Tsuge, H
通讯作者：
Tsuge, H