Localization and synthesis of the hormone-binding regions of the human thyrotropin receptor.
Localization and synthesis of the hormone-binding regions of the human thyrotropin receptor.
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人促甲状腺素受体激素结合区域的定位和合成。
DOI：
--
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发表时间：
1991
影响因子：
11.1
通讯作者：
S. Sakata
中科院分区：
文献类型：
--
作者：
M. Atassi;T. Manshouri;S. Sakata
人类促甲状腺激素（促甲状腺激素，TSH）受体（TSHR）的两个区域（残基12-44和308-364）是根据它们与黄体生成素/绒毛膜促性腺激素受体的序列不相似而选择的。研究了5种合成重叠肽（12-30、24-44、308-328、324-344和339-364）结合125i标记的人TSH （hTSH）、其分离的α和β亚基、牛TSH、羊TSH、人促黄体生成素和人促卵泡激素的能力。人TSHR肽12-30和324-344对人、牛和羊的TSH及其β链具有显著的结合活性。较低的结合活性存在于相邻的重叠肽中，可能是由于共享重叠对结合的贡献。TSH与这些肽结合的特异性被证实为它们不能结合人黄体生成素、人促卵泡激素和hTSH的α链。促甲状腺素不与牛血清白蛋白或与TSHR系统无关的肽对照结合。此外，hTSH与TSHR肽12-30和324-344的结合几乎完全（约90%）被兔抗hTSH抗体抑制，而不被抗无关蛋白的抗血清抑制。结论是，TSH与其受体的结合涉及广泛的接触，TSHR肽12-30和324-344含有TSH的特异性结合区，这些结合区可能是独立的位点，也可能是一个大结合位点内的两个面（亚位点）。
Two regions of human thyrotropin (thyroid-stimulating hormone, TSH) receptor (TSHR) (residues 12-44 and 308-364) were selected on the basis that they exhibit no sequence resemblance to luteinizing hormone/chorionic gonadotropin receptor. Five synthetic overlapping peptides (12-30, 24-44, 308-328, 324-344, and 339-364) were studied for their ability to bind 125I-labeled human TSH (hTSH), its isolated alpha and beta subunits, bovine TSH, ovine TSH, human luteinizing hormone, and human follicle-stimulating hormone. The human TSHR peptides 12-30 and 324-344 exhibited remarkable binding activity to human, bovine, and ovine TSH and to the beta chain of hTSH. Lower binding activity resided in the adjacent overlapping peptides, probably due to the contribution of the shared overlap to the binding. The specificity of TSH binding to these peptides was confirmed by their inability to bind human luteinizing hormone, human follicle-stimulating hormone, and the alpha chain of hTSH. Thyrotropins did not bind to bovine serum albumin or to peptide controls unrelated to the TSHR system. Furthermore, the binding of hTSH to TSHR peptides 12-30 and 324-344 was almost completely (approximately 90%) inhibited by rabbit antibodies against hTSH but not by antisera against unrelated proteins. It is concluded that the binding of TSH to its receptor involves extensive contacts and that the TSHR peptides 12-30 and 324-344 contain specific binding regions for TSH that might be either independent sites or two faces (subsites) within a large binding site.
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大鼠促甲状腺素β亚基染色体基因的组织和核苷酸序列分析。
DOI：
10.1089/dna.1986.5.299
发表时间：
1986
期刊：
DNA (Mary Ann Liebert, Inc.)
影响因子：
--
作者：
Croyle,ML;Bhattacharya,A;Gordon,DF;Maurer,RA
通讯作者：
Maurer,RA
加州鱼雷乙酰胆碱受体 α 链胞外部分的 α-金环蛇毒素结合区概况。
DOI：
10.1042/bj2480847
发表时间：
1987
期刊：
The Biochemical journal
影响因子：
--
作者：
Mulac-Jericevic,B;Atassi,MZ
通讯作者：
Atassi,MZ
乙酰胆碱受体-α-金环蛇毒素相互作用：通过肽-肽相互作用和受体腔的分子建模确定区域与区域的接触。
DOI：
10.1073/pnas.87.16.6156
发表时间：
1990
影响因子：
11.1
作者：
Ruan,KH;Spurlino,J;Quiocho,FA;Atassi,MZ
通讯作者：
Atassi,MZ
牛甲状腺切片与促甲状腺素一起孵育与百日咳毒素对腺苷二磷酸-核糖基鸟嘌呤核苷酸调节成分的能力降低有关。
DOI：
10.1210/mend-1-7-482
发表时间：
1987
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Ribeiro-Neto,F;Birnbaumer,L;Field,JB
通讯作者：
Field,JB
影响因子：
56.9
作者：
MCFARLAND, KC;SPRENGEL, R;SEEBURG, PH
通讯作者：
SEEBURG, PH