A rapid and scalable density gradient purification method for Plasmodium sporozoites.
A rapid and scalable density gradient purification method for Plasmodium sporozoites.
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DOI：
10.1186/1475-2875-11-421
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发表时间：
2012-12-17
期刊：
影响因子：
3
通讯作者：
Lindner SE
中科院分区：
文献类型：
--
作者：
Kennedy M;Fishbaugher ME;Vaughan AM;Patrapuvich R;Boonhok R;Yimamnuaychok N;Rezakhani N;Metzger P;Ponpuak M;Sattabongkot J;Kappe SH;Hume JC;Lindner SE
Malaria remains a major human health problem, with no licensed vaccine currently available. Malaria infections initiate when infectious Plasmodium sporozoites are transmitted by Anopheline mosquitoes during their blood meal. Investigations of the malaria sporozoite are, therefore, of clear medical importance. However, sporozoites can only be produced in and isolated from mosquitoes, and their isolation results in large amounts of accompanying mosquito debris and contaminating microbes. Here is described a discontinuous density gradient purification method for Plasmodium sporozoites that maintains parasite infectivity in vitro and in vivo and greatly reduces mosquito and microbial contaminants. This method provides clear advantages over previous approaches: it is rapid, requires no serum components, and can be scaled to purify >107 sporozoites with minimal operator involvement. Moreover, it can be effectively applied to both human (Plasmodium falciparum, Plasmodium vivax) and rodent (Plasmodium yoelii) infective species with excellent recovery rates. This novel method effectively purifies viable malaria sporozoites by greatly reducing contaminating mosquito debris and microbial burdens associated with parasite isolation. Large-scale preparations of purified sporozoites will allow for enhanced in vitro infections, proteomics, and biochemical characterizations. In conjunction with aseptic mosquito rearing techniques, this purification technique will also support production of live attenuated sporozoites for vaccination.
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影响因子：
30.3
作者：
Butler NS;Schmidt NW;Vaughan AM;Aly AS;Kappe SH;Harty JT
通讯作者：
Harty JT
影响因子：
2.1
作者：
BEAUDOIN, RL;STROME, CPA;TUBERGEN, TA
通讯作者：
TUBERGEN, TA
DOI：
10.1111/j.1550-7408.1973.tb03594.x
发表时间：
1973-01-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
KRETTLI, A;CHEN, DH;NUSSENZWEIG, RS
通讯作者：
NUSSENZWEIG, RS
影响因子：
6.7
作者：
Lasonder, Edwin;Janse, Chris J.;Stunnenberg, Hendrik G.
通讯作者：
Stunnenberg, Hendrik G.
影响因子：
2
作者：
TSUJI, M;MATTEI, D;ZAVALA, F
通讯作者：
ZAVALA, F