Efficacy of Different Encapsulation Techniques on the Viability and Stability of Diverse Phage under Simulated Gastric Conditions.
Efficacy of Different Encapsulation Techniques on the Viability and Stability of Diverse Phage under Simulated Gastric Conditions.
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DOI：
10.3390/microorganisms11102389
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发表时间：
2023-09-25
期刊：
影响因子：
4.5
通讯作者：
Atterbury RJ
中科院分区：
文献类型：
--
作者：
Dlamini SB;Gigante AM;Hooton SPT;Atterbury RJ
沙门氏菌在人类和牲畜中引起一系列疾病，具有相当大的公共卫生和经济重要性。广泛使用抗菌素，特别是在集约化生产的牲畜（如家禽和猪）中，可能导致耐多药沙门氏菌菌株的增加。替代疗法，如噬菌体，在用于减少牲畜肠道沙门氏菌携带时显示出了希望。然而，消化酶和单胃消化道的低pH值会显著降低噬菌体活力，影响治疗效果。本研究使用海藻酸盐-卡拉胶微胶囊（含CaCO3和不含CaCO3）来保护基因组多样化的五种沙门氏菌噬菌体免受模拟胃肠道疾病的影响。未受保护的噬菌体在pH < 3条件下暴露10分钟后均不能恢复。海藻酸盐-卡拉胶包封在pH 2 - 2.5下暴露10分钟后提高了噬菌体的活力，但在pH 2下暴露1小时后却没有。在配方中加入1% （w/v）的CaCO3，即使在pH 2下1小时后，噬菌体的损失也进一步降低到<0.5 log10 PFU/mL。在所有情况下，噬菌体在转移到中性pH（7.5）后有效地从微胶囊中释放出来，模拟进入十二指肠。综上所述，海藻酸-卡拉胶- caco3包封是一种很有前景的靶向肠道递送基因组多样性沙门氏菌噬菌体的方法。
Salmonella causes a range of diseases in humans and livestock of considerable public health and economic importance. Widespread antimicrobial use, particularly in intensively produced livestock (e.g., poultry and pigs) may contribute to the rise of multidrug-resistant Salmonella strains. Alternative treatments such as bacteriophages have shown promise when used to reduce the intestinal carriage of Salmonella in livestock. However, the digestive enzymes and low pH encountered in the monogastric GI tract can significantly reduce phage viability and impact therapeutic outcomes. This study deployed alginate–carrageenan microcapsules with and without CaCO3 to protect a genomically diverse set of five Salmonella bacteriophages from simulated gastrointestinal conditions. None of the unprotected phage could be recovered following exposure to pH < 3 for 10 min. Alginate–carrageenan encapsulation improved phage viability at pH 2–2.5 after exposure for 10 min, but not at pH 2 after 1 h. Including 1% (w/v) CaCO3 in the formulation further reduced phage loss to <0.5 log10 PFU/mL, even after 1 h at pH 2. In all cases, phage were efficiently released from the microcapsules following a shift to a neutral pH (7.5), simulating passage to the duodenum. In summary, alginate–carrageenan-CaCO3 encapsulation is a promising approach for targeted intestinal delivery of genomically diverse Salmonella bacteriophages.
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