In Vitro Design and Evaluation of Phage Cocktails Against Aeromonas salmonicida.
In Vitro Design and Evaluation of Phage Cocktails Against Aeromonas salmonicida.
复制标题
针对杀鲑气单胞菌的噬菌体混合物的体外设计和评估
DOI：
10.3389/fmicb.2018.01476
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发表时间：
2018
影响因子：
5.2
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Chen L;Yuan S;Liu Q;Mai G;Yang J;Deng D;Zhang B;Liu C;Ma Y
作为一种针对多重耐药细菌感染的替代方法，抗生素作为有效的治疗剂正在被越来越多地研究。在这里，旨在设计一个有效的噬菌体鸡尾酒对杀鲑气单胞菌感染，我们分离和表征5裂解A。杀鲑素、AS-szw、AS-yj、AS-zj、AS-sw和AS-gz。形态学和基因组学分析结果表明，这些病毒均属于尾状病毒目T4病毒属。它们对A.通过实验证实杀鲑属菌株。制备了一系列噬菌体混合物并进行了体外研究。我们观察到，组合AS-gz和AS-yj的混合物显示出比其他混合物和单独的混合物显著更高的抗微生物活性。考虑到AS-yj和AS-gz之间的基因组差异，我们的结果强调了噬菌体感染宿主的异质机制可能导致噬菌体协同杀死宿主。总之，我们的研究描述了一种策略，以开发一种有效的和有前途的噬菌体鸡尾酒作为治疗剂，以打击A。杀鲑感染，从而控制相关鱼类疾病的爆发。我们的研究表明，在体外调查到噬菌体是先决条件，以获得令人满意的噬菌体鸡尾酒在实践中应用之前。
As an alternative approach against multidrug-resistant bacterial infections, phages are now being increasingly investigated as effective therapeutic agents. Here, aiming to design an efficient phage cocktail against Aeromonas salmonicida infections, we isolated and characterized five lytic A. salmonicida phages, AS-szw, AS-yj, AS-zj, AS-sw, and AS-gz. The results of morphological and genomic analysis suggested that all these phages are affiliated to the T4virus genus of the Caudovirales order. Their heterogeneous lytic capacities against A. salmonicida strains were demonstrated by experiments. A series of phage cocktails were prepared and investigated in vitro. We observed that the cocktail combining AS-gz and AS-yj showed significantly higher antimicrobial activity than other cocktails and individual phages. Given the divergent genomes between the phages AS-yj and AS-gz, our results highlight that the heterogeneous mechanisms that phages use to infect their hosts likely lead to phage synergy in killing the host. Conclusively, our study described a strategy to develop an effective and promising phage cocktail as a therapeutic agent to combat A. salmonicida infections, and thereby to control the outbreak of relevant fish diseases. Our study suggests that in vitro investigations into phages are prerequisite to obtain satisfying phage cocktails prior to application in practice.
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DOI：
10.1080/21597081.2015.1096995
发表时间：
2016-01-01
期刊：
Bacteriophage
影响因子：
--
作者：
Mapes, Abigail C;Trautner, Barbara W;Ramig, Robert F
通讯作者：
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影响因子：
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作者：
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影响因子：
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Llagostera M
影响因子：
14.9
作者：
Besemer, J;Lomsadze, A;Borodovsky, M
通讯作者：
Borodovsky, M
DOI：
10.1007/978-1-4939-6536-6_3
发表时间：
2017-01-01
期刊：
CAMPYLOBACTER JEJUNI: METHODS AND PROTOCOLS
影响因子：
--
作者：
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通讯作者：
Brondsted, Lone