Prediction of the preoperative chemoradiotherapy response for rectal cancer by peripheral blood lymphocyte subsets.
Prediction of the preoperative chemoradiotherapy response for rectal cancer by peripheral blood lymphocyte subsets.
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DOI：
10.1186/s12957-014-0418-0
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发表时间：
2015-02-07
影响因子：
3.2
通讯作者：
Watanabe T
中科院分区：
文献类型：
--
作者：
Tada N;Kawai K;Tsuno NH;Ishihara S;Yamaguchi H;Sunami E;Kitayama J;Oba K;Watanabe T
虽然新辅助放化疗（CRT）已成为手术前降低局部晚期直肠癌分期的标准程序，但预测CRT反应的标志物尚未完全确定。本研究的目的是确定CRT反应的预测因素，特别是关注外周血白细胞亚群。共有45例连续诊断为原发性直肠癌的患者前瞻性入组，接受CRT治疗，随后进行根治性切除。采用流式细胞术分析CRT前后外周血中各淋巴细胞亚群的数量。根据CRT的病理反应将患者分为高反应组（Hi-R）和低反应组（Lo-R）。Hi-R病例的CRT前淋巴细胞（p = 0.018）、T淋巴细胞（p = 0.009）和辅助性T淋巴细胞（Th淋巴细胞，p = 0.015）数量显著高于Lo-R病例。CRT前T淋巴细胞数受试者工作特征曲线的曲线下面积（AUC）为0.733，最佳临界值为1196/μl，敏感性为76.5%，特异性为67.8%，阳性预测值为59.1%，阴性预测值为82.6%。在多变量分析中，CRT前Th淋巴细胞和细胞毒性淋巴细胞的数量都是CRT高反应的独立预测因素。除了CRT的直接细胞毒性外，最近的研究表明，CRT诱导的免疫宿主反应也有助于CRT诱导的肿瘤消退。我们的研究结果表明，在预测CRT在结直肠癌患者的反应循环T淋巴细胞的潜在作用。
Although neoadjuvant chemoradiotherapy (CRT) has become a standard procedure to downstage locally advanced rectal cancer prior to surgery, markers to predict the response to CRT have not been fully identified. The aim of this study was to identify predictive factors of response to CRT, especially focusing on peripheral blood leukocyte subsets. A total of 45 consecutive patients diagnosed with primary rectal cancer were prospectively enrolled and received CRT followed by curative resection. The numbers of each lymphocyte subset in peripheral blood pre- and post-CRT were analyzed using flow cytometry. According to the pathological response to CRT, patients were classified into high (Hi-R) and low (Lo-R) response groups. Hi-R cases had significantly higher numbers of pre-CRT lymphocytes (p = 0.018), T lymphocytes (p = 0.009) and helper T lymphocytes (Th lymphocytes, p = 0.015) compared to the Lo-R cases. With the receiver-operating characteristic curve for numbers of pre-CRT T lymphocytes, the area under the curve (AUC) was 0.733, and the optimal cutoff value was 1196/μl, with 76.5% sensitivity, 67.8% specificity, 59.1% positive and 82.6% negative predictive values. The numbers of pre-CRT Th lymphocytes and cytotoxic lymphocytes were both independent predictors of the high CRT response in the multivariate analysis. In addition to the direct cytotoxicity of CRT, recent studies have demonstrated the induction of an immunological host response, which also contributed to the tumor regression induced by CRT. Our result suggested the potential role of circulating T lymphocytes in predicting the response to CRT in colorectal cancer patients.
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影响因子：
7.4
作者：
Milne K;Alexander C;Webb JR;Sun W;Dillon K;Kalloger SE;Gilks CB;Clarke B;Köbel M;Nelson BH
通讯作者：
Nelson BH
DOI：
10.1006/bbrc.2000.2287
发表时间：
2000-03-16
影响因子：
3.1
作者：
Huber, MA;Kraut, N;Peter, RU
通讯作者：
Peter, RU
影响因子：
158.5
作者：
Sauer, R;Becker, H;Raab, R
通讯作者：
Raab, R
影响因子：
4.7
作者：
Demaria S;Formenti SC
通讯作者：
Formenti SC
影响因子：
3.8
作者：
Kitayama J;Yasuda K;Kawai K;Sunami E;Nagawa H
通讯作者：
Nagawa H