Descending vasa recta endothelium is an electrical syncytium.
Descending vasa recta endothelium is an electrical syncytium.
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直肠降血管内皮是电合胞体。
DOI：
10.1152/ajpregu.00261.2006
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Pallone,ThomasL
中科院分区：
文献类型：
--
作者：
Zhang,Qingli;Cao,Chunhua;Mangano,Michael;Zhang,Zhong;Silldorff,ErikP;Lee-Kwon,Whaseon;Payne,Kristie;Pallone,ThomasL
我们研究了直肠血管降支(DVR)的缝隙连接偶联。DVR内皮细胞或周细胞去极化以记录相关的电容瞬变。几乎所有的内皮细胞和一些周细胞都表现出长时间的瞬变，持续10-30ms。甘草酮(100μM)和18β甘草次酸(18βGRA；100μM)可显著缩短血管内皮细胞的瞬变时间。甘草酮和庚醇(2 MM)延长后可使周细胞电容瞬变时间减少。将荧光黄(LY；2 mm)透析到内皮细胞和周细胞的细胞质中。LY沿内皮单分子层扩散，而在大多数周细胞中，它局限于单个细胞。在某些周细胞中，可观察到复杂的LY铺展模式。在200μm处监测双(1，3-二巴比妥酸)-三亚甲基草醇[DiBAC_4]的荧光，用电压钳去极化。40 mV的内皮去极化伴随着26.1±5.5 mV的DiBAC4荧光变化。DIBAC4荧光在18βGRA后或周细胞去极化后无变化。同样，18βGRA或庚醇可减弱机械扰动DVR壁引起的胞浆内钙离子反应。连接蛋白(Cx)免疫组织化学染色显示血管内皮细胞以线状Cx40和Cx43为主，而Cx37染色呈平滑肌肌动蛋白阳性的周细胞。我们认为DVR内皮细胞是一个电合体，DVR周细胞中存在缝隙连接偶联，但不太明显。
We examined gap junction coupling of descending vasa recta (DVR). DVR endothelial cells or pericytes were depolarized to record the associated capacitance transients. Virtually all endothelia and some pericytes exhibited prolonged transients lasting 10–30 ms. Carbenoxolone (100 μM) and 18β-glycyrrhetinic acid (18βGRA; 100 μM) markedly shortened the endothelial transients. Carbenoxolone and heptanol (2 mM) reduced the pericyte capacitance transients when they were prolonged. Lucifer yellow (LY; 2 mM) was dialyzed into the cytoplasm of endothelial cells and pericytes. LY spread diffusely along the endothelial monolayer, whereas in most pericytes, it was confined to a single cell. In some pericytes, complex patterns of LY spreading were observed. DVR cells were depolarized by voltage clamp as fluorescence of bis(1,3-dibarbituric acid)-trimethine oxanol [DiBAC4] was monitored ∼200 μm away. A 40-mV endothelial depolarization was accompanied by a 26.1 ± 5.5-mV change in DiBAC4fluorescence. DiBAC4fluorescence did not change after 18βGRA or when pericytes were depolarized. Similarly, propagated cytoplasmic Ca2+responses arising from mechanical perturbation of the DVR wall were attenuated by 18βGRA or heptanol. Connexin (Cx) immunostaining showed predominant linear Cx40 and Cx43 in endothelia, whereas Cx37 stained smooth muscle actin-positive pericytes. We conclude that the DVR endothelium is an electrical syncytium and that gap junction coupling in DVR pericytes exists but is less pronounced.
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影响因子：
3.6
作者：
R. Taugner;H. Kirchheim;W. Forssmann
通讯作者：
R. Taugner;H. Kirchheim;W. Forssmann
影响因子：
5
作者：
Harris, D;Martin, PEM;Randall, MD
通讯作者：
Randall, MD
DOI：
10.1152/nips.01430.2002
发表时间：
2003-06
期刊：
News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society
影响因子：
--
作者：
T. W. White
通讯作者：
T. W. White
肾髓质周细胞内存在平滑肌 α-肌动蛋白的证据。
DOI：
--
发表时间：
1997
期刊：
American Journal of Physiology. Regulatory Integrative and Comparative Physiology
影响因子：
--
作者：
F. Park;D. Mattson;L. A. Roberts;A. Cowley
通讯作者：
A. Cowley
间隙连接蛋白connexin 37在大鼠气管和肺动脉平滑肌细胞中的分布。
影响因子：
--
作者：
K. Nakamura;T. Inai;K. Nakamura;Y. Shibata
通讯作者：
Y. Shibata