Potent immune response against HIV-1 and protection from virus challenge in hu-PBL-SCID mice immunized with inactivated virus-pulsed dendritic cells generated in the presence of IFN-alpha.
Potent immune response against HIV-1 and protection from virus challenge in hu-PBL-SCID mice immunized with inactivated virus-pulsed dendritic cells generated in the presence of IFN-alpha.
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DOI：
10.1084/jem.20021924
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发表时间：
2003-07-21
期刊：
影响因子：
--
通讯作者：
Belardelli F
中科院分区：
文献类型：
--
作者：
Lapenta C;Santini SM;Logozzi M;Spada M;Andreotti M;Di Pucchio T;Parlato S;Belardelli F
艾滋病研究的一个主要挑战是开发能够诱导针对HIV-1的免疫应答的体液和细胞臂的治疗性疫苗策略。在这项工作中，我们评估了用醛硫醇-2灭活的HIV-1脉冲的DC在用人PBL重建的SCID小鼠（hu-PBL-SCID小鼠）中诱导保护性抗病毒人免疫应答的能力。用单核细胞暴露于GM-CSF/IFN-α（IFN-DCs）后产生的DCs免疫hu-PBL-SCID小鼠，并用灭活的HIV-1脉冲，导致人抗HIV-1抗体的显著诱导，这与血清中抗HIV中和活性的检测相关。通过IFN-γ Elispot分析测定，该疫苗接种方案还促进了针对HIV-1的人CD 8 + T细胞应答的产生。值得注意的是，当用抗原脉冲的IFN-DC免疫的hu-PBL-SCID小鼠感染HIV-1时，与对照动物相比，观察到病毒感染的抑制。这些结果表明，IFN-DCs与灭活的HIV-1脉冲可以代表一个有价值的方法免疫干预HIV-1感染的患者。
A major challenge of AIDS research is the development of therapeutic vaccine strategies capable of inducing the humoral and cellular arms of the immune responses against HIV-1. In this work, we evaluated the capability of DCs pulsed with aldrithiol-2–inactivated HIV-1 in inducing a protective antiviral human immune response in SCID mice reconstituted with human PBL (hu-PBL-SCID mice). Immunization of hu-PBL-SCID mice with DCs generated after exposure of monocytes to GM-CSF/IFN-α (IFN-DCs) and pulsed with inactivated HIV-1 resulted in a marked induction of human anti–HIV-1 antibodies, which was associated with the detection of anti-HIV neutralizing activity in the serum. This vaccination schedule also promoted the generation of a human CD8+ T cell response against HIV-1, as measured by IFN-γ Elispot analysis. Notably, when the hu-PBL-SCID mice immunized with antigen-pulsed IFN-DCs were infected with HIV-1, inhibition of virus infection was observed as compared with control animals. These results suggest that IFN-DCs pulsed with inactivated HIV-1 can represent a valuable approach of immune intervention in HIV-1–infected patients.
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影响因子：
82.9
作者：
Buseyne, F;Le Gall, S;Schwartz, O
通讯作者：
Schwartz, O
I型干扰素是单核细胞衍生的树突状细胞发育和体外和HU-PBL-SCID小鼠活性的强大佐剂。
影响因子：
15.3
作者：
Santini, S M;Lapenta, C;Logozzi, M;Parlato, S;Spada, M;Di Pucchio, T;Belardelli, F
通讯作者：
Belardelli, F
影响因子：
4.4
作者：
Santodonato, L;D'Agostino, G;Ferrantini, M
通讯作者：
Ferrantini, M
影响因子：
56.9
作者：
Ogg, GS;Jin, X;McMichael, AJ
通讯作者：
McMichael, AJ
影响因子：
32.4
作者：
Poignard, P;Sabbe, R;Burtons, DR
通讯作者：
Burtons, DR