Comparative Analysis of Ciliary Membranes and Ectosomes.
Comparative Analysis of Ciliary Membranes and Ectosomes.
复制标题
睫状膜和胞外体的比较分析
DOI：
10.1016/j.cub.2016.09.055
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发表时间：
2016-12-19
期刊：
影响因子：
9.2
通讯作者：
Huang, Kaiyao
中科院分区：
文献类型：
--
作者：
Long, Huan;Zhang, Fan;Xu, Nannan;Liu, Gai;Diener, Dennis R.;Rosenbaum, Joel L.;Huang, Kaiyao
原发纤毛和活动纤毛作为细胞天线，接收来自环境的信号，随后激活对细胞稳态和分化至关重要的信号通路。最近对绿藻衣藻和线虫秀丽隐杆线虫的研究表明，外体可以从纤毛中释放出来，并可以介导细胞间的通讯。为了更好地了解鞭毛外泌体的功能，我们将它们的蛋白质组成与它们的来源鞭毛膜的蛋白质组成进行了比较。鞭毛释放的外泌体具有独特的蛋白质组成，富含鞭毛膜蛋白、蛋白酶、运输所需的内体分选复合体蛋白（ESCRT）、小gtpase和泛素化蛋白。实时成像显示，在配子激活过程中鞭毛释放的外体中富含escrt相关蛋白（PDCD6）。我们设计了一种灵敏、快速的检测方法，利用荧光素酶与PDCD6和突变的泛素融合来监测外泌体的释放。当细胞进行鞭毛吸收时，胞外体释放增加。两种escrt相关蛋白PDCD6和VPS4的敲低，在鞭毛缩短和缩短过程中减弱了外泌体的释放。这些数据表明ESCRT蛋白介导衣藻外体释放，从而影响鞭毛缩短。此外，纤毛外体中普遍存在凝集素和泛素化蛋白等受体，表明它们参与细胞信号传导和纤毛蛋白的转换。Long等人分离出鞭毛外泌体，并证明鞭毛外泌体具有独特的蛋白质组成，特别是富含运输所需的内体分选复合物（ESCRT）和泛素化蛋白。敲低ESCRT蛋白可减弱鞭毛外体的释放，从而影响鞭毛缩短。
Primary and motile cilia/flagella function as cellular antennae, receiving signals from the environment, and subsequently activating signaling pathways that are critical for cellular homeostasis and differentiation. Recent work with the green alga Chlamydomonas and the nematode C. elegans demonstrated that ectosomes can be released from the cilium and can mediate the intercellular communication. To better understand the function of flagellar ectosomes, we have compared their protein composition to that of the flagellar membrane from which they are derived. Ectosomes released from flagella have a unique protein composition, being enriched in a subset of flagellar membrane proteins, proteases, proteins from the endosomal sorting complex required for transport (ESCRT), small GTPases, and ubiquitinated proteins. Live imaging showed that an ESCRT-related protein (PDCD6) was enriched in ectosomes released from flagella during gamete activation. We devised a sensitive and rapid assay to monitor ectosome release using luciferase fused to PDCD6 and a mutated ubiquitin. Ectosome release increased when cells underwent flagellar resorption. Knockdown of two ESCRT-related proteins, PDCD6 and VPS4, attenuated ectosome release during flagellar shortening and shortening was slowed. These data suggest that the ESCRT proteins mediate ectosome release and thereby influence flagellar shortening in Chlamydomonas. In addition, the prevalence of receptors such as agglutinin and ubiquitinated proteins in ciliary ectosomes suggests that they are involved in cell signaling and turnover of ciliary proteins. Long et al. isolate flagellar ectosomes and demonstrate that flagellar ectosomes have a unique protein composition, especially being enriched in endosomal sorting complex required for transport (ESCRT) and ubiquitinated proteins. Knock down of ESCRT proteins attenuates the flagellar ectosome release and thereby influences flagellar shortening.
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DOI：
10.1016/j.cub.2015.10.057
发表时间：
2015-12-21
期刊：
Current biology : CB
影响因子：
--
作者：
Wang J;Kaletsky R;Silva M;Williams A;Haas LA;Androwski RJ;Landis JN;Patrick C;Rashid A;Santiago-Martinez D;Gravato-Nobre M;Hodgkin J;Hall DH;Murphy CT;Barr MM
通讯作者：
Barr MM
莱茵衣藻人工microRNA系统的快速构建与筛选
影响因子：
7.2
作者：
Hu, Jinlu;Deng, Xuan;Huang, Kaiyao
通讯作者：
Huang, Kaiyao
DOI：
10.1111/j.1600-0854.2011.01242.x
发表时间：
2011-10
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Shields SB;Piper RC
通讯作者：
Piper RC
影响因子：
11.8
作者：
Pan, JM;Snell, WJ
通讯作者：
Snell, WJ
影响因子：
56.9
作者：
Jimenez, Ana Joaquina;Maiuri, Paolo;Perez, Franck
通讯作者：
Perez, Franck