An ear punch model for studying the effect of radiation on wound healing.
An ear punch model for studying the effect of radiation on wound healing.
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DOI：
10.3109/09553002.2011.568575
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发表时间：
2011-08
影响因子：
2.6
通讯作者：
Chao NJ
中科院分区：
文献类型：
--
作者：
Deoliveira D;Jiao Y;Ross JR;Corbin K;Xiao Q;Toncheva G;Anderson-Evans C;Yoshizumi TT;Chen BJ;Chao NJ
放射和创伤联合损伤代表了一个主要的临床挑战，因为协同相互作用导致的发病率和死亡率高于任何一种损伤单独产生的。本研究的目的是建立一个小鼠耳穿孔模型，以研究辐射对愈合伤口的生理机制。通过在MRL/MpJ小鼠的耳廓中的2 mm手术穿孔来诱导手术伤口。拍摄伤口照片，并通过图像分析定量耳穿孔伤口的大小。局部辐射到耳朵是由正电压X射线辐照器使用一个特殊构造的夹具，屏蔽身体的其他部分。使用该模型，我们证明了对伤口区域的局部辐射以剂量依赖性方式显著延迟了耳穿孔伤口的愈合。增加亚致死量全身照射（7戈伊）进一步延迟了耳穿孔伤口的愈合。这些结果在C57 BL/6小鼠中得到了复制;然而，MRL/MpJ小鼠的伤口愈合加速。提示小鼠耳穿孔伤模型是一种较好的研究放创复合伤的模型。
Radiation and wound combined injury represents a major clinical challenge because of the synergistic interactions that lead to higher morbidity and mortality than either insult would produce singly. The purpose of this study was to develop a mouse ear punch model to study the physiological mechanisms underlying radiation effects on healing wounds. Surgical wounds were induced by a 2 mm surgical punch in the ear pinnae of MRL/MpJ mice. Photographs of the wounds were taken and the sizes of the ear punch wounds were quantified by image analysis. Local radiation to the ear was delivered by orthovoltage X-ray irradiator using a specially constructed jig that shields the other parts of body. Using this model, we demonstrated that local radiation to the wound area significantly delayed the healing of ear punch wounds in a dose-dependent fashion. The addition of sublethal whole body irradiation (7 Gy) further delayed the healing of ear punch wounds. These results were replicated in C57BL/6 mice; however, wound healing in MRL/MpJ mice was accelerated. These data indicate that the mouse ear punch model is a valuable model to study radiation and wound combined injury.
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影响因子：
3.1
作者：
Dormand, Emma-Louise;Banwell, Paul E;Goodacre, Timothy E E
通讯作者：
Goodacre, Timothy E E
影响因子：
3.6
作者：
BAXTER, H;DRUMMOND, JA;RANDALL, RG
通讯作者：
RANDALL, RG
影响因子：
1
作者：
Charles, MW
通讯作者：
Charles, MW
DOI：
10.3109/02841868109130187
发表时间：
1981-01-01
期刊：
ACTA RADIOLOGICA ONCOLOGY
影响因子：
--
作者：
LEDNEY, GD;STEWART, DA;SHEEHY, PA
通讯作者：
SHEEHY, PA
影响因子：
8.2
作者：
Thomson, S. E.;McLennan, S. V.;Twigg, S. M.
通讯作者：
Twigg, S. M.