Magnesium alleviates aluminum toxicity by promoting polar auxin transport and distribution and root alkalization in the root apex in populus
Magnesium alleviates aluminum toxicity by promoting polar auxin transport and distribution and root alkalization in the root apex in populus
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镁通过促进杨树根尖的极性生长素运输和分布以及根部碱化来减轻铝毒性
DOI：
10.1007/s11104-020-04459-7
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发表时间：
2020-02
期刊：
影响因子：
4.9
通讯作者：
Li Nannan
中科院分区：
文献类型：
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作者：
Zhang Zhen;Liu Dunyi;Meng Hongjun;Li Shengting;Wang Shufeng;Xiao Zhongchun;Sun Juanjuan;Chang Longran;Luo Keming;Li Nannan
目的杨树具有耐高浓度铝胁迫的能力。然而，镁减轻杨树铝毒性的机制尚不清楚。方法采用电生理分析、荧光染色等方法检测杨树在高浓度铝中毒胁迫下根表面的pH梯度、根端对Mg和Al的吸收。此外，还检测了生长素在根尖的分布和运输动力学。结果在本研究中，我们发现充足的Mg供应减轻了高浓度铝对杨树根系生长的毒性。Mg提高了杨树根表面的pH梯度，导致根过渡区碱化，阻止了铝对根伸长的毒害。利用DR5:GFP报告基因对杨树根尖生长素分布进行了检测。铝毒性抑制了根过渡区极性生长素的运输和分布，Mg的补充部分缓解了极性生长素的运输和分布。利用生长素转运体突变体pin-formed 2 （pin2）进行的进一步电生理实验表明，Mg通过pin2为基础的极性生长素转运减轻了铝的毒性，该转运调节了过渡区根表面的碱化。转录组学分析和利用mrs2突变体进行的酵母互补实验也揭示了Mg、生长素和Al之间的相同关系。结论本研究表明Mg促进了生长素的极性运输和分布，从而提高了根表面pH的调节，从而进一步减轻了Al的毒性。本研究结果部分阐明了Mg减轻高浓度铝对木本植物毒性的作用机制。
Aims Populus can tolerant high concentration Al stress. However, the mechanisms of Mg alleviation to Al toxicity in populus remain unknown. Methods In the present study, adequate Mg was supplied to poplar under high concentration Al toxicity stress, and the pH gradients of root surface, Mg and Al uptake from root apex were detected using electrophysiological analysis, fluorescence staining, etc. Furthermore, auxin distribution and transport dynamic kinetics in root tips were also detected. Results In the present study, we found that adequate supply of Mg alleviated high concentration Al toxicity to populus root growth. Mg promoted the pH gradients of root surface in populus, leading to alkalization in the root transition zone, which prevented Al toxicity to root elongation. The auxin distribution in root tips of populus was detected using DR5:GFP reporter. The transport and distribution of polar auxin in root transition zone were inhibited by Al toxicity, but partially alleviated by Mg supply. Further electrophysiological experiments using auxin transporter mutant pin-formed 2 (pin2) showed that Mg alleviated Al toxicity by PIN2-based polar auxin transport, which regulated root surface alkalization in the transition zone. Transcriptomic analysis and yeast complementation experiments using mrs2 mutants also reveals same relationships among Mg, auxin and Al. Conclusions The present study suggests that Mg promotes polar auxin transport and distribution, which leads to the elevated root surface pH regulation, and further alleviates the Al toxicity. Our results partially clarify the mechanism of Mg in alleviating the toxicity of high concentration Al in woody plants.
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
T. Kinraide;P. Ryan;L. Kochian
通讯作者：
T. Kinraide;P. Ryan;L. Kochian
DOI：
10.1016/j.plantsci.2009.01.002
发表时间：
2009-04
期刊：
Plant science : an international journal of experimental plant biology
影响因子：
--
作者：
B. Pitann;T. Kranz;Karl H. Mühling
通讯作者：
B. Pitann;T. Kranz;Karl H. Mühling
MATE 转运蛋白的全基因组分析和杨树铝抗性的分子表征
影响因子：
6.9
作者：
Li N;Meng H;Xing H;Liang L;Zhao X;Luo K
通讯作者：
Luo K
影响因子：
4.3
作者：
Shen, RF;Ma, JF;Iwashita, T
通讯作者：
Iwashita, T
影响因子：
7.4
作者：
DELHAIZE, E;RYAN, PR
通讯作者：
RYAN, PR