Comparison of human fibroblast ECM-related gene expression on elastic three-dimensional substrates relative to two-dimensional films of the same material.
Comparison of human fibroblast ECM-related gene expression on elastic three-dimensional substrates relative to two-dimensional films of the same material.
复制标题
相对于相同材料的二维薄膜，弹性三维基底上的人成纤维细胞 ECM 相关基因表达的比较。
DOI：
10.1016/s0142-9612(03)00368-5
复制
发表时间：
2003
期刊：
影响因子：
14
通讯作者：
Tresco,PatrickA
中科院分区：
文献类型：
--
作者：
Webb,Ken;Li,Wenhua;Hitchcock,RobertW;Smeal,RoyM;Gray,StevenD;Tresco,PatrickA
由具有约70%的内部孔隙率和27.4±2.76KPa的弹性模量的市售聚氨酯制造三维弹性基底，并检查其在声带组织工程中的适用性。使用免疫组织化学，生物力学测试，和RT-PCR，我们研究了人成纤维细胞的活力，分布和细胞外基质相关的基因表达基板内长达4周的时间。我们发现，细胞能够以高活力定殖于5 mm宽× 3 mm深× 20 mm长的基底的整个体积。组织学横截面显示广泛的细胞外基质沉积在细胞周围和基质的整个孔结构中，其由纤连蛋白和I型胶原组成。细胞接种基质显示出比未接种对照组显著更高的弹性模量，类似于天然组织。细胞生长从二维到三维培养的转移导致ECM相关基因表达的变化，这与细胞迁移减少和组织形成增加一致。我们发现，成纤维细胞培养在三维基板表达的mRNA水平显着较高的弹性蛋白和纤调蛋白，而表达的MMP-1和透明质酸酶的mRNA水平显着较低，相对于二维基板相同的材料。结果表明，三维多孔，Tecoflex衍生的弹性生物材料可能是合适的工程声带组织的基板。
Three-dimensional elastic substrates were fabricated from a commercially available polyurethane with an internal porosity of approximately 70% and elastic modulus of 27.4±2.76KPa and examined for suitability in vocal fold tissue engineering. Using immunohistochemistry, biomechanical testing, and RT-PCR; we examined human fibroblast viability, distribution and extracellular matrix related gene expression within substrates for periods up to 4 weeks. We found that cells were capable of colonizing the entire volume of a 5mm wide×3mm deep×20mm long substrate at high viability. Histological cross-sections showed extensive extracellular matrix deposited around the cells and throughout the pore structure of the substrates, which consisted of fibronectin and type I collagen. Cell seeded substrates displayed a significantly higher elastic modulus than unseeded controls similar to native tissue. The transfer of cell growth from two-dimensional to three-dimensional culture resulted in changes in ECM-related gene expression consistent with decreasing cell migration and increasing tissue formation. We found that fibroblasts cultured in three-dimensional substrates expressed significantly higher levels of mRNA for elastin and fibromodulin, while expressing significantly lower levels of mRNA for MMP-1 and hyaluronidase relative to two-dimensional substrates of the same material. The results suggest that three-dimensionally porous, Tecoflex-derived elastic biomaterials may be suitable substrates for engineering vocal fold tissue.
登录
查看更多内容
在疤痕声带注射胶原蛋白
DOI：
--
发表时间：
1987
期刊：
影响因子：
--
作者：
C. Ford;D. Bless
通讯作者：
D. Bless
DOI：
10.1002/jbm.820270104
发表时间：
1993-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
FREED, LE;MARQUIS, JC;LANGER, R
通讯作者：
LANGER, R
弹性体基质上的骨骼肌生成：对组织工程的影响。
DOI：
--
发表时间：
1998
期刊：
Journal of Biomaterials Science. Polymer Edition
影响因子：
--
作者：
M. M. Mulder;R. Hitchcock;P. Tresco
通讯作者：
P. Tresco
绳内注射Teflon®治疗发音障碍135例
DOI：
--
发表时间：
1973
期刊：
Annals of Otology, Rhinology and Laryngology
影响因子：
--
作者：
H. Dedo;Robert D. Urrea;L. Lawson
通讯作者：
L. Lawson
影响因子：
56.9
作者：
Niklason, LE;Gao, J;Langer, R
通讯作者：
Langer, R