Interspecific studies of circadian genes period and timeless in Drosophila.
Interspecific studies of circadian genes period and timeless in Drosophila.
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DOI：
10.1016/j.gene.2018.01.020
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发表时间：
2018-03-30
期刊：
影响因子：
3.5
通讯作者：
Kyriacou CP
中科院分区：
文献类型：
--
作者：
Noreen S;Pegoraro M;Nouroz F;Tauber E;Kyriacou CP
利用种间Clock基因转化在遗传心律失常的黑腹果蝇宿主中挽救Clock功能的水平来研究相互作用的Clock蛋白之间可能的分子间协同进化。其中，PER和TIM是生物钟反馈环中两个重要的负调节因子。我们将Per或Tim基因转化为相应的心律失常突变株(Per01或tim01)，观察到50%的节律性，但与对照相比，活动节律的周期要么更长(D.Posoblura-per)，要么短于24 h(D.puoblcura-Tim)。通过将两个转基因同时导入双突变体中，我们观察到一对半合子转基因(~24 h)挽救了活性节律的周期。在此期间，这些苍蝇也表现出了更理想的温度补偿水平。根据LD 12：12，这些苍蝇的活动轮廓类似于假黑腹果蝇，没有早晨的预期，以及非常显著的傍晚活动节奏高峰期。这些观察结果与TIM和PER至少在活动节律的昼夜节律期间形成了一个异种共同进化模块的观点是一致的。然而，两个转基因的存在降低了节律性的强度，所以尽管在某些性状上观察到了PER和TIM之间存在共同进化的证据，但对其他性状却并非如此。为了了解Period和其伴侣之间永恒的分子间协同进化机制，我们将来自假黑斑潜蝇的转基因导入到黑腹隐翅虫缺失突变体中。转化子的节律性低于50%，运动节律的周期也很长(PERP为26-31h)或很短(TIMPS为21-22h)。在双突变体黑腹果蝇中，两个异种转基因共同作用并不能显着增加节律性水平，但运动活动的平均周期有显着改善(~24小时)。这两个转化子在运动活动期的温度补偿也得到了改善。经Western blotts和qPCR检测，证实了这些转基因的表达。
The level of rescue of clock function in genetically arrhythmic Drosophila melanogaster hosts using interspecific clock gene transformation was used to study the putative intermolecular coevolution between interacting clock proteins. Among them PER and TIM are the two important negative regulators of the circadian clock feedback loop. We transformed either the D. pseudoobscura per or tim transgenes into the corresponding arrhythmic D. melanogaster mutant (per01 or tim01) and observed >50% rhythmicity but the period of activity rhythm was either longer (D. pseudoobscura-per) or shorter than 24 h (D. pseudoobscura-tim) compared to controls. By introducing both transgenes simultaneously into double mutants, we observed that the period of the activity rhythm was rescued by the pair of hemizygous transgenes (~24 h). These flies also showed a more optimal level of temperature compensation for the period. Under LD 12:12 these flies have a D. pseudoobscura like activity profile with the absence of morning anticipation as well as a very prominent earlier evening peak of activity rhythm. These observation are consistent with the view that TIM and PER form a heterospecific coevolved module at least for the circadian period of activity rhythms. However the strength of rhythmicity was reduced by having both transgenes present, so while evidence for a coevolution between PER and TIM is observed for some characters it is not for others. To understand the mechanism of intermolecular coevolution between period and its partner timeless, transgenes from D. pseudoobscura were introduced into D. melanogaster null mutants. The single D. melanogaster transformants containing either per or tim from D. pseudoobscura displayed less than 50% rhythmicity and the period of locomotor activity rhythms was also either very long (26-31h for perps) or very short (21-22h for timps). The two heterospecific transgenes together in the double mutant D. melanogaster flies could not show a significant increase in levels of rhythmicity however a significant improvement in the average period of locomotor activity was obtained (~24h). Temperature compensation in the period of locomotor activity was also improved in these double tranformants. The Western blots and qPCR confirmed the expression of these transgenes.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
16.2
作者：
Low, Kwang Huei;Lim, Cecilia;Ederyl, Isaac
通讯作者：
Ederyl, Isaac
DOI：
10.1073/pnas.0402591101
发表时间：
2004-06-15
影响因子：
11.1
作者：
Fraser, HB;Hirsh, AE;Eisen, MB
通讯作者：
Eisen, MB
影响因子：
11
作者：
Allebrandt, K. V.;Amin, N.;Roenneberg, T.
通讯作者：
Roenneberg, T.
影响因子：
64.8
作者：
Grima, B;Chèlot, E;Rouyer, F
通讯作者：
Rouyer, F