Hedgehog signaling controls mouth opening in the amphioxus.
Hedgehog signaling controls mouth opening in the amphioxus.
复制标题
刺猬信号控制文昌鱼的张口
DOI：
10.1186/s40851-021-00186-8
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发表时间：
2021-12-24
影响因子：
2.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Hu G;Li G;Wang Y
文昌鱼（amphioxus）幼虫嘴的左侧位置长期以来一直吸引着研究人员。尽管文昌鱼口腔发育的基本重要性，其发育的分子调控几乎是未知的。在我们之前的研究中，我们发现文昌鱼的突变导致文昌鱼不张嘴，这表明Hh信号是文昌鱼张嘴形成的必要条件。然而，由于hhmutant也表现出早期左右（LR）模式的缺陷，目前尚不清楚张嘴的丧失是由Hh缺乏直接影响还是其对LR建立的影响的次要影响。结果我们发现，敲除文文鱼Hh信号通路的另一个关键成分theSmogene会导致嘴巴不张开，但对LR不对称发育没有影响。Hh信号的上调导致嘴的大小急剧增加。smomation不能影响LR的发育是由于smo在文昌鱼卵和卵裂期胚胎中的高母体表达。在smomutants中，pou4和pax2/5/8在原始口腔部位的表达在张嘴前没有改变。结论基于上述结果和我们之前的研究，我们认为Hh信号是文昌鱼口腔形成的必要条件，并且Hh介导的口腔发育调控是口腔特异性的。我们的数据表明，Hh信号在文昌鱼中以与脊椎动物相似的方式调节嘴的形成，表明Hh信号在嘴的形成中起着保守的作用。
IntroductionThe left-sided position of the mouth in amphioxus larvae has fascinated researchers for a long time. Despite the fundamental importance of mouth development in the amphioxus, the molecular regulation of its development is almost unknown. In our previous study, we showed thatHhmutation in the amphioxus leads to no mouth opening, indicating a requirement of Hh signaling for amphioxus mouth formation. Nevertheless, since theHhmutant also exhibits defects in early left-right (LR) patterning, it remains currently unknown whether the loss of mouth opening is affected directly by Hh deficiency or a secondary effect of its influence on LR establishment.ResultsWe demonstrated that knockout of theSmogene, another key component of the Hh signaling pathway, in the amphioxus resulted in the absence of mouth opening, but caused no effects on LR asymmetry development. Upregulation of Hh signaling led to a dramatic increase in mouth size. The inability ofSmomutation to affect LR development is due toSmo’s high maternal expression in amphioxus eggs and cleavage-stage embryos. InSmomutants,Pou4andPax2/5/8expression at the primordial oral site is not altered before mouth opening.ConclusionsBased on these results and our previous study, we conclude that Hh signal is necessary for amphioxus mouth formation and that the Hh-mediated regulation of mouth development is specific to the mouth. Our data suggest that Hh signaling regulates mouth formation in the amphioxus in a similar way as that in vertebrates, indicating the conserved role of Hh signaling in mouth formation.
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影响因子：
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作者：
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