Manganese dioxide nanosheets induce mitochondrial toxicity in fish gill epithelial cells.
Manganese dioxide nanosheets induce mitochondrial toxicity in fish gill epithelial cells.
复制标题
二氧化锰纳米片在鱼鳃上皮细胞中诱导线粒体毒性。
DOI：
10.1080/17435390.2021.1874562
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发表时间：
2021-04
期刊：
影响因子：
5
通讯作者：
Kane AB
中科院分区：
文献类型：
--
作者：
Browning CL;Green A;Gray EP;Hurt R;Kane AB
工程2D纳米材料的开发和生产正在呈指数级增长，增加了它们释放到水生环境中的风险。最近的一项研究表明，正在开发用于能源和生物医学应用的2D MnO2纳米片在与生物还原剂相互作用时溶解，导致鱼鳃细胞内细胞内谷胱甘肽水平的耗尽。然而，关于它们的毒性和与亚细胞器的相互作用知之甚少。为了解决这一差距，我们研究了细胞吸收，细胞毒性和线粒体的影响，2D二氧化锰纳米片使用体外鱼鳃细胞系，以代表目标组织的虹鳟鱼，淡水指示物种。这些数据表明，细胞摄取的MnO2纳米片进入溶酶体和潜在的机制内的溶酶体室溶解。MnO2纳米片在亚细胞毒性剂量下诱导严重的线粒体功能障碍。定量，单细胞荧光成像显示线粒体分裂和受损的线粒体膜电位MnO2纳米片曝光。海马细胞呼吸的分析表明，MnO2纳米片抑制基础呼吸，最大呼吸和鳃细胞的备用呼吸能力，表明线粒体功能障碍和细胞呼吸活性降低。MnO2纳米片暴露也抑制ATP的产生，进一步支持线粒体功能和细胞呼吸的抑制。总之，这些观察结果表明，2D MnO2纳米片损害了鳃细胞对能量需求或长期应激的反应能力。最后，我们的数据表明2D MnO 2纳米片和它们的微粒对应物的毒性存在显着差异。这证明了在进行安全评估时考虑2D纳米材料独特物理特性的重要性。
The development and production of engineered 2D nanomaterials are expanding exponentially, increasing the risk of their release into the aquatic environment. A recent study showed 2D MnO2 nanosheets, under development for energy and biomedical applications, dissolve upon interaction with biological reducing agents, resulting in depletion of intracellular glutathione levels within fish gill cells. However, little is known concerning their toxicity and interactions with subcellular organelles. To address this gap, we examined cellular uptake, cytotoxicity and mitochondrial effects of 2D MnO2 nanosheets using an in vitro fish gill cell line to represent a target tissue of rainbow trout, a freshwater indicator species. The data demonstrate cellular uptake of MnO2 nanosheets into lysosomes and potential mechanisms of dissolution within the lysosomal compartment. MnO2 nanosheets induced severe mitochondrial dysfunction at sub-cytotoxic doses. Quantitative, single cell fluorescent imaging revealed mitochondrial fission and impaired mitochondrial membrane potential following MnO2 nanosheet exposure. Seahorse analyses for cellular respiration revealed that MnO2 nanosheets inhibited basal respiration, maximal respiration and the spare respiratory capacity of gill cells, indicating mitochondrial dysfunction and reduced cellular respiratory activity. MnO2 nanosheet exposure also inhibited ATP production, further supporting the suppression of mitochondrial function and cellular respiration. Together, these observations indicate that 2D MnO2 nanosheets impair the ability of gill cells to respond to energy demands or prolonged stress. Finally, our data demonstrate significant differences in the toxicity of the 2D MnO2 nanosheets and their microparticle counterparts. This exemplifies the importance of considering the unique physical characteristics of 2D nanomaterials when conducting safety assessments.
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影响因子：
29.4
作者：
Chen, Yu;Ye, Delai;Wang, Lianzhou
通讯作者：
Wang, Lianzhou
影响因子：
2.8
作者：
Dawson, Neal J.;Millet, Caroline;Metcalfe, Neil B.
通讯作者：
Metcalfe, Neil B.
DOI：
10.1166/jcsb.2013.1057
发表时间：
2013-09-01
期刊：
JOURNAL OF COLLOID SCIENCE AND BIOTECHNOLOGY
影响因子：
--
作者：
Islam, Md. Aminul;Rahman, M. Muhibur
通讯作者：
Rahman, M. Muhibur
影响因子：
7.3
作者：
Gray, Evan P.;Browning, Cynthia L.;Hurt, Robert H.
通讯作者：
Hurt, Robert H.
影响因子：
3.5
作者：
Bonora, Massimo;Patergnani, Simone;Pinton, Paolo
通讯作者：
Pinton, Paolo