Temperature-Inducible Transgenic EDS1 and PAD4 in Arabidopsis Confer an Enhanced Disease Resistance at Elevated Temperature.
Temperature-Inducible Transgenic EDS1 and PAD4 in Arabidopsis Confer an Enhanced Disease Resistance at Elevated Temperature.
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拟南芥中温度诱导的转基因 EDS1 和 PAD4 可在高温下增强抗病性
DOI：
10.3390/plants10061258
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发表时间：
2021-06-21
期刊：
影响因子：
--
通讯作者：
Cui H
中科院分区：
文献类型：
--
作者：
Leng J;Tu W;Hou Y;Cui H
温度是影响植物病害发生的重要环境因子之一。高温有利于许多植物病害的爆发，这威胁着粮食安全，并成为沿着气候变化和全球变暖的一个大问题。在这里，我们发现关键免疫调节因子EDS 1和PAD 4在拟南芥中的同时组成型表达显著增强了对有毒细菌病原体假单胞菌的抗性。番茄在升高的温度;然而，自身免疫相关的生长迟缓也观察到这些植物在正常温度。为了平衡这种生长-防御权衡，我们产生了在来自HSP 70基因的温度敏感启动子的控制下双表达EDS 1和PAD 4基因的转基因植物，HSP 70基因的表达在升高的温度下高度诱导。与组成型过表达株系不同，proHSP 70-EP转基因株系在高温下表现出对细菌病原体的抗性增强，而在正常条件下没有生长缺陷。因此，本研究提供了一个潜在的策略，植物的遗传操作，以应对同时非生物和生物胁迫。
Temperature is one of the most important environmental factors greatly affecting plant disease development. High temperature favors outbreaks of many plant diseases, which threaten food security and turn to be a big issue along with climate change and global warming. Here, we found that concurrent constitutive expression of the key immune regulators EDS1 and PAD4 in Arabidopsis significantly enhanced resistance to virulent bacterial pathogen Pseudomonas syringae pv. tomato at elevated temperature; however, autoimmunity-related growth retardation was also observed on these plants at a normal temperature. To balance this growth-defense trade-off, we generated transgenic plants dual expressing EDS1 and PAD4 genes under the control of a thermo-sensitive promoter from the HSP70 gene, whose expression is highly induced at an elevated temperature. Unlike constitutive overexpression lines, the proHSP70-EP transgenic lines exhibited enhanced resistance to bacterial pathogens at an elevated temperature without growth defects at normal condition. Thus, this study provides a potential strategy for genetic manipulation of plants to deal with the simultaneous abiotic and biotic stresses.
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影响因子：
16.6
作者：
Cheng, Cheng;Gao, Xiquan;Feng, Baomin;Sheen, Jen;Shan, Libo;He, Ping
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影响因子：
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3.5
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Clarke, JD;Aarts, N;Parker, JE
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