Evaluation of clarithromycin lactobionate as a novel chiral selector for enantiomeric separation of basic drugs in capillary electrophoresis
Evaluation of clarithromycin lactobionate as a novel chiral selector for enantiomeric separation of basic drugs in capillary electrophoresis
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乳糖酸克拉霉素作为新型手性选择剂用于毛细管电泳碱性药物对映体分离的评价
DOI：
10.1002/elps.201000630
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发表时间：
2011-07
期刊：
影响因子：
2.9
通讯作者：
Chen, Bin
中科院分区：
文献类型：
--
作者：
Yu, Tao;Du, Yingxiang;Chen, Bin
如今，大环抗生素的对映体分离应用越来越多。在毛细管电泳 (CE) 中用作手性选择剂的大环抗生素包括安沙霉素和糖肽。大环内酯类是另一类重要的大环抗生素，最近被报道为一种新型手性选择剂。在本研究中，首次研究了属于大环内酯类抗生素的乳糖克拉霉素 (CL) 作为 CE 中新型手性选择剂用于多种碱性药物对映体分离的潜力。据观察，CL 可以很好地分离美托洛尔、阿替洛尔、普萘洛尔、比索洛尔、艾司洛尔、利托君和氨氯地平的对映体，以及拉贝洛尔和奈福泮的部分对映体拆分。此外，CL还具有在溶剂中溶解度高、粘度低、紫外吸收极弱等优点。在本研究过程中，发现碱性药物的迁移时间和对映体分离都受到几个实验参数的影响，例如选择器浓度、BGE 的组成和 pH 值、有机改性剂的类型和浓度以及施加的电压。因此，系统地研究了这些因素的影响，并使用 12.5mM 硼砂缓冲液和 50%v/v 甲醇、60mM CL 和 20kV 外加电压，在 7.3-7.5 的缓冲液 pH 范围内实现了所研究药物的满意的对映体分离。此外，本文还通过统计产品和服务解决方案（SPSS）进一步研究了所研究参数的影响比较。
Nowadays, macrocyclic antibiotics are presenting an increasing number of enantioseparation applications. The macrocyclic antibiotics used as chiral selectors in capillary electrophoresis (CE) include the ansamycins and the glycopeptides. The macrolides, another important class of macrocyclic antibiotics, have been reported as a new type of chiral selectors recently. In this study, clarithromycin lactobionate (CL), belonging to the group of macrolide antibiotics, was first investigated for its potential as a novel chiral selector in CE for enantiomeric separation of several basic drugs. As observed, CL allowed excellent separation of the enantiomers of metoprolol, atenolol, propranolol, bisoprolol, esmolol, ritodrine, and amlodipine, as well as partial enantioresolution of labetalol and nefopam. In addition, CL possesses advantages such as high solubility and low viscosity in the solvent and very weak UV absorption. In the course of this study, it was found that both migration times and enantioseparation of the basic drugs were influenced by several experimental parameters, e.g. selector concentration, the composition and pH of the BGE, the type and concentration of organic modifier, and applied voltage. Thus, the effects of these factors were systematically investigated, and satisfactory enantioseparations of the studied drugs were achieved at the buffer pH range of 7.3–7.5 using 12.5 mM borax buffer with 50% v/v methanol, 60 mM CL, and 20 kV applied voltage. Moreover, comparison of the influences of the studied parameters was further investigated by means of Statistical Product and Service Solutions (SPSS) in this article.
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DOI：
10.1016/j.chroma.2005.07.082
发表时间：
2005-11
期刊：
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影响因子：
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作者：
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DOI：
10.1016/0021-9673(95)00608-p
发表时间：
1995-11
期刊：
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