Generation and analysis of an improved Foxg1-IRES-Cre driver mouse line.
Generation and analysis of an improved Foxg1-IRES-Cre driver mouse line.
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DOI：
10.1016/j.ydbio.2016.02.011
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发表时间：
2016-04-01
影响因子：
2.7
通讯作者：
O'Leary DDM
中科院分区：
文献类型：
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作者：
Kawaguchi D;Sahara S;Zembrzycki A;O'Leary DDM
Foxg 1的表达在早期胚胎中高度局限于端脑和其他头部结构。这种表达模式已被利用来产生条件性敲除小鼠，基于广泛使用的Foxg 1-Cre敲入系（Foxg 1 tm 1（cre）Skm），其中Foxg 1编码区被Cre基因取代。然而，由于两个原因，该品系的实用性受到严重阻碍：（1）Foxg 1-Cre小鼠显示出异位和不可预测的Cre活性，以及（2）Foxg 1单倍不足可产生神经发育表型。为了克服这些问题，我们已经产生了一种新的Foxg 1-IRES-Cre敲入小鼠系，其中IRES-Cre盒插入Foxg 1基因座的3′UTR中，从而保留了内源性Foxg 1编码区和3′UTR中的非翻译基因调控序列，包括最近发现的microRNA靶向位点。我们进一步证明，新的Foxg 1-IRES-Cre线显示一致的Cre活性模式，重演的内源性Foxg 1表达在胚胎和出生后阶段，而不会导致皮质发育缺陷。我们的结论是，新的Foxg 1-IRES-Cre小鼠系是一种独特的和先进的工具，用于研究从早期胚胎阶段开始参与端脑和其他Foxg 1表达区域发育的基因。
Foxg1 expression is highly restricted to the telencephalon and other head structures in the early embryo. This expression pattern has been exploited to generate conditional knockout mice, based on a widely used Foxg1-Cre knock-in line (Foxg1tm1(cre)Skm), in which the Foxg1 coding region was replaced by the Cre gene. The utility of this line, however, is severely hampered for two reasons: (1) Foxg1-Cre mice display ectopic and unpredictable Cre activity, and (2) Foxg1 haploinsufficiency can produce neurodevelopmental phenotypes. To overcome these issues, we have generated a new Foxg1-IRES-Cre knock-in mouse line, in which an IRES-Cre cassette was inserted in the 3′UTR of Foxg1 locus, thus preserving the endogenous Foxg1 coding region and un-translated gene regulatory sequences in the 3′UTR, including recently discovered microRNA targeting sites. We further demonstrate that the new Foxg1-IRES-Cre line displays consistent Cre activity patterns that recapitulated the endogenous Foxg1 expression at embryonic and postnatal stages without causing defects in cortical development. We conclude that the new Foxg1-IRES-Cre mouse line is a unique and advanced tool for studying genes involved in the development of the telencephalon and other Foxg1-expressing regions starting from early embryonic stages.
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影响因子：
3.7
作者：
Dou, CL;Li, S;Lai, ES
通讯作者：
Lai, ES
影响因子：
16.2
作者：
Gil-Sanz, Cristina;Espinosa, Ana;Mueller, Ulrich
通讯作者：
Mueller, Ulrich
影响因子：
4.6
作者：
Fuccillo, M;Rallu, M;Fishell, G
通讯作者：
Fishell, G
影响因子：
25
作者：
Chou SJ;Perez-Garcia CG;Kroll TT;O'Leary DD
通讯作者：
O'Leary DD
影响因子：
16.6
作者：
通讯作者：
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