A novel RNA polymerase-binding protein that interacts with a sigma-factor docking site.
A novel RNA polymerase-binding protein that interacts with a sigma-factor docking site.
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DOI：
10.1111/mmi.13724
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发表时间：
2017-08
影响因子：
3.6
通讯作者：
Losick R
中科院分区：
文献类型：
--
作者：
Wang Erickson AF;Deighan P;Chen S;Barrasso K;Garcia CP;Martínez-Lumbreras S;Alfano C;Krysztofinska EM;Thapaliya A;Camp AH;Isaacson RL;Hochschild A;Losick R
枯草芽孢杆菌的产孢是由一系列可选择的RNA聚合酶sigma因子控制的。我们之前发现了一种小蛋白Fin，它是在产孢sigma因子σF的控制下产生的，可以产生一个抑制σF导向基因转录的负反馈回路。缺失的细胞在孢子形成方面存在缺陷，并且表现出更高的σ f导向基因转录水平。基于下拉实验、化学交联、细菌双杂交实验和核磁共振化学位移分析，我们现在报道Fin与RNA聚合酶结合，特别是与β '亚基的卷曲区域结合。螺旋形线圈是RNA聚合酶上sigma因子的对接位点，有证据表明，Fin和σF与RNA聚合酶的结合是互斥的。我们认为Fin的作用机制不同于经典的sigma因子拮抗剂（抗σ因子），它直接结合到目标sigma因子上，阻止其与RNA聚合酶的结合，而通过竞争与β '卷曲线圈的结合来抑制σF。在枯草芽孢杆菌孢子的发育过程中，一系列与RNA聚合酶相关的sigma因子直接影响基因的表达。我们报道了一种新的rnap结合蛋白Fin在产孢过程中产生，通过竞争σF的2区（Rg2）与RNA聚合酶β ‘亚基（β ’ CC）的卷曲区域的结合，抑制了产孢sigma因子σF的功能。
Sporulation in Bacillus subtilis is governed by a cascade of alternative RNA polymerase sigma factors. We previously identified a small protein Fin that is produced under the control of the sporulation sigma factor σF to create a negative feedback loop that inhibits σF-directed gene transcription. Cells deleted for fin are defective for spore formation and exhibit increased levels of σF-directed gene transcription. Based on pull-down experiments, chemical crosslinking, bacterial two-hybrid experiments, and nuclear magnetic resonance chemical shift analysis, we now report that Fin binds to RNA polymerase and specifically to the coiled-coil region of the β’ subunit. The coiled-coil is a docking site for sigma factors on RNA polymerase, and evidence is presented that the binding of Fin and σF to RNA polymerase is mutually exclusive. We propose that Fin functions by a mechanism distinct from that of classic sigma factor antagonists (anti-σ factors), which bind directly to a target sigma factor to prevent its association with RNA polymerase, and instead functions to inhibit σF by competing for binding to the β’ coiled-coil. During the developmental process of sporulation in Bacillus subtilis a cascade of alternative sigma factors associate with RNA polymerase to direct gene expression. We report that Fin, a novel RNAP-binding protein produced during sporulation, inhibits the function of the sporulation sigma factor σF by competing with the binding of region 2 (Rg2) of σF to the coiled-coiled region of the β’ subunit of RNA polymerase (β’ CC).
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影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
DOI：
10.1073/pnas.0400923101
发表时间：
2004-03-30
影响因子：
11.1
作者：
Gregory, BD;Nickels, BE;Hochschild, A
通讯作者：
Hochschild, A
影响因子：
5.6
作者：
Baxter, Kimberly;Lee, Jennifer;Hinton, Deborah M.
通讯作者：
Hinton, Deborah M.
影响因子：
3.6
作者：
Patrick, Joyce E.;Kearns, Daniel B.
通讯作者：
Kearns, Daniel B.
DOI：
10.1073/pnas.54.3.704
发表时间：
1965-01-01
影响因子：
11.1
作者：
SCHAEFFER, P;MILLET, J;AUBERT, JP
通讯作者：
AUBERT, JP