Cloning and functional characterization of auxin receptor TIR1 in Gossypium hirsutum
Cloning and functional characterization of auxin receptor TIR1 in Gossypium hirsutum
复制标题
陆地棉生长素受体 TIR1 的克隆及功能表征
DOI：
10.1007/s11738-020-03198-7
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发表时间：
2021-02
影响因子：
2.6
通讯作者：
Zhang Genfa
中科院分区：
文献类型：
--
作者：
Wu Yu;Ming Hainan;Xu Jing;Bing Jie;Chen Qing;Wang Qing;Li Hongjie;Zhang Genfa
运输抑制剂反应1（TransportInhibitorResponse 1，TIR 1）是植物生长素的核受体，属于F-box家族蛋白。它在生长素信号转导途径和生长素应答基因的表达中是必不可少的。生长素是植物体内最重要的激素，对棉花胚珠和纤维的发育有重要影响。研究生长素信号转导途径对棉花纤维发育的影响，可以从根本上解决纤维产量和品质问题。克隆了G.通过同源克隆和cDNA末端快速扩增（RACE）技术获得了陆地棉TIR 1基因。GhTIR 1与拟南芥的同源性为64.8%。为了研究GhTIR 1序列的功能、亚细胞定位和特异性定位，我们构建了GhTIR 1及其N端或C端缺失突变基因与GFP的融合表达载体。通过瞬时转化拟南芥原生质体和稳定转化烟草BY-2细胞系，GhTIR 1通过N-末端1-197个氨基酸序列定位于细胞核。GhTIR 1在棉花不同器官、胚珠和不同发育时期棉纤维细胞中的表达模式表明，GhTIR 1在棉纤维发育的起始期和伸长期高表达。这些结果表明，GhTIR 1在棉纤维的起始和伸长过程中起重要作用，尤其是在棉纤维细胞初生纤维的形成过程中。
Transport inhibitor response 1 (TIR1), as an F-box family protein, is a nucleus receptor of auxin in plants. It is essential in the auxin signal transduction pathway and the expression of auxin-responsive genes. As the most important hormone in plants, auxin has a great influence on the ovule and fiber development of cotton (Gossypium hirsutum L.). Studies on the effect of auxin signal transduction pathway on cotton fiber development can fundamentally solve the problem of fiber yield and quality. The cDNA sequence of G. hirsutum TIR1 was obtained through homologous cloning and rapid-amplification of cDNA ends (RACE). The homology between GhTIR1 and that in Arabidopsis thaliana was 64.8%. To study the functions, subcellular localization, and the specific localization of GhTIR1 sequence, we constructed the fusion expression vector of GhTIR1 and its N-terminal or C-terminal deletion mutant genes with GFP. By transiently transforming Arabidopsis protoplasts and stably transforming Nicotiana tabacum BY-2 cell lines, GhTIR1 was localized in the nucleus through the N-terminal 1–197 amino acid sequence. The expression patterns of GhTIR1 in different organs and ovule and cotton fiber cells at different developmental stages showed that GhTIR1 was highly expressed in the initial and elongation stages of cotton fiber development. These results indicate that GhTIR1 plays an important role in the initiation and elongation of cotton fibers, especially the formation of primary fibers of cotton fiber cells.
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影响因子：
14.8
作者：
Uchida N;Takahashi K;Iwasaki R;Yamada R;Yoshimura M;Endo TA;Kimura S;Zhang H;Nomoto M;Tada Y;Kinoshita T;Itami K;Hagihara S;Torii KU
通讯作者：
Torii KU
DOI：
10.1073/pnas.1717649115
发表时间：
2018-04-24
影响因子：
11.1
作者：
Vaseva II;Qudeimat E;Potuschak T;Du Y;Genschik P;Vandenbussche F;Van Der Straeten D
通讯作者：
Van Der Straeten D
DOI：
10.1016/j.bbaexp.2004.06.008
发表时间：
2004-09-17
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Kim, HJ;Triplett, BA
通讯作者：
Triplett, BA
影响因子：
64.5
作者：
HEASMAN, J;CRAWFORD, A;WYLIE, C
通讯作者：
WYLIE, C
影响因子：
4
作者：
Lee, Sarah;Sundaram, Shanthy;Armitage, Lynne;Evans, John P.;Hawkes, Tim;Kepinski, Stefan;Ferro, Noel;Napier, Richard M.
通讯作者：
Napier, Richard M.