Investigating the Structural Compaction of Biomolecules Upon Transition to the Gas-Phase Using ESI-TWIMS-MS.
Investigating the Structural Compaction of Biomolecules Upon Transition to the Gas-Phase Using ESI-TWIMS-MS.
复制标题
DOI：
10.1007/s13361-017-1689-9
复制
发表时间：
2017-09
影响因子：
3.2
通讯作者：
Ashcroft AE
中科院分区：
文献类型：
--
作者：
Devine PWA;Fisher HC;Calabrese AN;Whelan F;Higazi DR;Potts JR;Lowe DC;Radford SE;Ashcroft AE
从离子迁移谱-质谱（IMS-MS）分析获得的碰撞截面（CCS）测量通常提供关于蛋白质的大小和形状的有用信息，并且可以通过建模程序来补充。然而，在气相分析过程中，某些蛋白质在多大程度上保持天然样构象，特别是具有动态或扩展区域的蛋白质，一直存在一些问题。在这里，我们已经测量了一系列生物分子的CCS，包括不同序列、大小和稳定性的非球状蛋白质和RNA。使用行波IMS-MS，我们表明，对于研究的蛋白质，测量的CCS偏离预测CCS值的基础上，目前可用的结构显着。结果表明，这些蛋白质崩溃到不同程度上不同的延长结构过渡到气相。比较两个RNA的质量相似，但不同的解决方案的结构，我们表明，这些生物分子也可能容易受到气相压缩。总之，这些结果表明，在预测基于CCS数据的RNA以及具有非球状折叠的蛋白质的结构模型时需要谨慎。本文的在线版本（doi：10.1007/s13361-017-1689-9）包含补充材料，可供授权用户使用。
Collision cross-section (CCS) measurements obtained from ion mobility spectrometry-mass spectrometry (IMS-MS) analyses often provide useful information concerning a protein’s size and shape and can be complemented by modeling procedures. However, there have been some concerns about the extent to which certain proteins maintain a native-like conformation during the gas-phase analysis, especially proteins with dynamic or extended regions. Here we have measured the CCSs of a range of biomolecules including non-globular proteins and RNAs of different sequence, size, and stability. Using traveling wave IMS-MS, we show that for the proteins studied, the measured CCS deviates significantly from predicted CCS values based upon currently available structures. The results presented indicate that these proteins collapse to different extents varying on their elongated structures upon transition into the gas-phase. Comparing two RNAs of similar mass but different solution structures, we show that these biomolecules may also be susceptible to gas-phase compaction. Together, the results suggest that caution is needed when predicting structural models based on CCS data for RNAs as well as proteins with non-globular folds. ᅟ The online version of this article (doi:10.1007/s13361-017-1689-9) contains supplementary material, which is available to authorized users.
登录
查看更多内容
DOI：
10.1016/j.str.2010.08.012
发表时间：
2010-11-10
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Gatzeva-Topalova PZ;Warner LR;Pardi A;Sousa MC
通讯作者：
Sousa MC
影响因子：
16.6
作者：
Gruszka, Dominika T.;Whelan, Fiona;Farrance, Oliver E.;Fung, Herman K. H.;Paci, Emanuele;Jeffries, Cy M.;Svergun, Dmitri I.;Baldock, Clair;Baumann, Christoph G.;Brockwell, David J.;Potts, Jennifer R.;Clarke, Jane
通讯作者：
Clarke, Jane
影响因子：
5.6
作者：
Harris, LJ;Skaletsky, E;McPherson, A
通讯作者：
McPherson, A
影响因子：
1.8
作者：
Baker, Erin Shammel;Bernstein, Summer L.;Bowers, Michael T.
通讯作者：
Bowers, Michael T.
影响因子：
16.6
作者：
Jenner, Matthew;Ellis, Jacqueline;Oldham, Neil J.
通讯作者：
Oldham, Neil J.