Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria.
Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria.
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PSB29/THF1的结构及其与蓝细菌中光系统II修复的FTSH蛋白酶复合物的关联。
DOI：
10.1098/rstb.2016.0394
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发表时间：
2017-09-26
期刊：
影响因子：
--
通讯作者：
Nixon PJ
中科院分区：
文献类型：
--
作者：
Bec Ková M;Yu J;Krynická V;Kozlo A;Shao S;Koník P;Komenda J;Murray JW;Nixon PJ
增强作物光合作用的一种策略是提高其修复光系统II（PSII）的能力，以响应光的不可逆损伤。尽管类囊体包埋的FtsH蛋白酶复合物在修复过程中选择性降解PSII亚基的关键作用，很少有人知道参与调节FtsH表达的因素。在这里，我们显示使用蓝藻集胞藻属PCC 6803的Psb 29亚基，最初确定为His标记的PSII制剂的一个次要组成部分，物理相互作用与FtsH复合物在体内，并需要参与PSII修复的FtsH 2/FtsH 3异源寡聚复合物的正常积累。我们使用X-射线晶体学表明，Psb 29从细长热聚球藻有一个独特的折叠组成的螺旋束和一个扩展的C-末端螺旋，并包含一个高度保守的区域，可能参与结合到FtsH。类似的相互作用可能发生在拟南芥叶绿体之间的Psb 29同源物，称为THF 1，和FTSH 2/FTSH 5复合物。Psb 29/THF 1直接参与FtsH的积累有助于解释为什么THF 1是病原体感染诱导的植物过敏反应过程中的靶标。通过THF 1下调FtsH功能和PSII修复周期将有助于活性氧的产生、叶绿体功能的丧失和细胞死亡。这篇文章是主题问题“增强作物光合作用：改进目标”的一部分。
One strategy for enhancing photosynthesis in crop plants is to improve their ability to repair photosystem II (PSII) in response to irreversible damage by light. Despite the pivotal role of thylakoid-embedded FtsH protease complexes in the selective degradation of PSII subunits during repair, little is known about the factors involved in regulating FtsH expression. Here we show using the cyanobacterium Synechocystis sp. PCC 6803 that the Psb29 subunit, originally identified as a minor component of His-tagged PSII preparations, physically interacts with FtsH complexes in vivo and is required for normal accumulation of the FtsH2/FtsH3 hetero-oligomeric complex involved in PSII repair. We show using X-ray crystallography that Psb29 from Thermosynechococcus elongatus has a unique fold consisting of a helical bundle and an extended C-terminal helix and contains a highly conserved region that might be involved in binding to FtsH. A similar interaction is likely to occur in Arabidopsis chloroplasts between the Psb29 homologue, termed THF1, and the FTSH2/FTSH5 complex. The direct involvement of Psb29/THF1 in FtsH accumulation helps explain why THF1 is a target during the hypersensitive response in plants induced by pathogen infection. Downregulating FtsH function and the PSII repair cycle via THF1 would contribute to the production of reactive oxygen species, the loss of chloroplast function and cell death. This article is part of the themed issue ‘Enhancing photosynthesis in crop plants: targets for improvement’.
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影响因子：
10.7
作者：
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通讯作者：
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DOI：
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发表时间：
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期刊：
Bioinformatics (Oxford, England)
影响因子：
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作者：
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通讯作者：
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影响因子：
10.7
作者：
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通讯作者：
Standley DM
DOI：
10.1107/s0907444909042073
发表时间：
2010-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Chen VB;Arendall WB 3rd;Headd JJ;Keedy DA;Immormino RM;Kapral GJ;Murray LW;Richardson JS;Richardson DC
通讯作者：
Richardson DC