Variation in ligand responses of the bitter taste receptors TAS2R1 and TAS2R4 among New World monkeys.
Variation in ligand responses of the bitter taste receptors TAS2R1 and TAS2R4 among New World monkeys.
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DOI：
10.1186/s12862-016-0783-0
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发表时间：
2016-10-12
影响因子：
3.4
通讯作者：
Imai H
中科院分区：
文献类型：
--
作者：
Tsutsui K;Otoh M;Sakurai K;Suzuki-Hashido N;Hayakawa T;Misaka T;Ishimaru Y;Aureli F;Melin AD;Kawamura S;Imai H
新世界猴（NWM）是独特的，因为它们在色觉和摄食行为方面表现出显着的种间差异，使它们成为研究感觉生态学的极好模型。然而，目前还不清楚非视觉感官是否与摄食生态共同变化，尤其是味觉，预计味觉在食物选择中不可或缺。由舌头中的苦味受体（TAS 2 R）介导的苦味有助于生物体避免摄入食物中的潜在有毒物质。在这项研究中，我们通过在HEK 293 T细胞中异源表达和钙成像比较了五种NWM的TAS 2 R的配体敏感性。我们发现TAS 2 R1和TAS 2 R4直系同源物在NWM物种中分别对秋水仙碱和樟脑的敏感性不同。然后，我们重建了NWM TAS 2 R1和TAS 2 R4的祖先受体，测量了配体敏感性的进化转变，并确定了第62位残基的氨基酸替换是绒猴TAS 2 R4对秋水仙碱的高敏感性的原因。我们的研究结果提供了一个基础，了解不同的摄食生态NWM之间的苦味。本文的在线版本（doi：10.1186/s12862-016-0783-0）包含补充材料，可供授权用户使用。
New World monkeys (NWMs) are unique in that they exhibit remarkable interspecific variation in color vision and feeding behavior, making them an excellent model for studying sensory ecology. However, it is largely unknown whether non-visual senses co-vary with feeding ecology, especially gustation, which is expected to be indispensable in food selection. Bitter taste, which is mediated by bitter taste receptors (TAS2Rs) in the tongue, helps organisms avoid ingesting potentially toxic substances in food. In this study, we compared the ligand sensitivities of the TAS2Rs of five species of NWMs by heterologous expression in HEK293T cells and calcium imaging. We found that TAS2R1 and TAS2R4 orthologs differ in sensitivity among the NWM species for colchicine and camphor, respectively. We then reconstructed the ancestral receptors of NWM TAS2R1 and TAS2R4, measured the evolutionary shift in ligand sensitivity, and identified the amino acid replacement at residue 62 as responsible for the high sensitivity of marmoset TAS2R4 to colchicine. Our results provide a basis for understanding the differences in feeding ecology among NWMs with respect to bitter taste. The online version of this article (doi:10.1186/s12862-016-0783-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
与抗肿瘤药物结合的人平滑受体的结构。
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
2.5
作者：
Matsushita, Yuka;Oota, Hiroki;Welker, Barbara J.;Pavelka, Mary S.;Kawamura, Shoji
通讯作者：
Kawamura, Shoji
影响因子：
2.4
作者：
Levenson, David H.;Fernandez-Duque, Eduardo;Jacobs, Gerald H.
通讯作者：
Jacobs, Gerald H.
影响因子：
3.5
作者：
Meyerhof, Wolfgang;Batram, Claudia;Behrens, Maik
通讯作者：
Behrens, Maik