Age-related changes in glial cells of dopamine midbrain subregions in rhesus monkeys.
Age-related changes in glial cells of dopamine midbrain subregions in rhesus monkeys.
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DOI：
10.1016/j.neurobiolaging.2008.07.006
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发表时间：
2010-06
影响因子：
4.2
通讯作者：
Collier, Timothy J.
中科院分区：
文献类型：
--
作者：
Kanaan, Nicholas M.;Kordower, Jeffrey H.;Collier, Timothy J.
关键词：
衰老仍然是帕金森病(PD)发生的最大危险因素，PD患者中脑DA神经元变性存在选择性易损性。通过跟踪正常衰老相关的变化，强调区域特异性，可以研究涉及选择性易损性和抗变性的因素。为此，我们试图确定恒河猴小胶质细胞和星形胶质细胞的年龄相关变化是否具有区域特异性，提示参与了可能与帕金森病发病相关的变性易感性的区域差异。通过使用无偏向体视学估计胶质细胞数量，评估激活过程中上调的蛋白质的荧光强度，以及对形态进行评级，来测量中脑DA亚区的胶质细胞增殖症。随着年龄的增长，小胶质细胞的染色强度增加，并向更活跃的形态转变，特别是在脆弱的黑质腹侧层(VtSN)。星形胶质细胞在任何方面都没有表现出与年龄相关的变化，这与区域脆弱性的参与是一致的。我们的结果表明，增龄与VtSN中的慢性轻度炎症有关，这可能使这些DA神经元更容易退化。
Aging remains the strongest risk factor for developing Parkinson’s disease (PD), and there is selective vulnerability in midbrain DA neuron degeneration in PD. By tracking normal aging-related changes with an emphasis on regional specificity, factors involved in selective vulnerability and resistance to degeneration can be studied. Towards this end, we sought to determine whether age-related changes in microglia and astrocytes in rhesus monkeys are region-specific, suggestive of involvement in regional differences in vulnerability to degeneration that may be relevant to PD pathogenesis. Gliosis in midbrain DA subregions was measured by estimating glia number using unbiased stereology, assessing fluorescence intensity for proteins upregulated during activation, and rating morphology. With normal aging, microglia exhibited increased staining intensity and a shift to more activated morphologies preferentially in the vulnerable substantia nigra-ventral tier (vtSN). Astrocytes did not exhibit age-related changes consistent with an involvement in regional vulnerability in any measure. Our results suggest advancing age is associated with chronic mild inflammation in the vtSN, which may render these DA neurons more vulnerable to degeneration.
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影响因子：
--
作者：
GEINISMAN, Y;BONDAREFF, W;DODGE, JT
通讯作者：
DODGE, JT
影响因子：
2
作者：
Gundersen, HJG;Jensen, EBV;Nielsen, J
通讯作者：
Nielsen, J
影响因子：
4.2
作者：
HANSEN, LA;ARMSTRONG, DM;TERRY, RD
通讯作者：
TERRY, RD
影响因子：
6.1
作者：
Chu, Yaping;Kordower, Jeffrey H.
通讯作者：
Kordower, Jeffrey H.
影响因子：
6.2
作者：
BEACH, TG;WALKER, R;MCGEER, EG
通讯作者：
MCGEER, EG