TroCCL4, a CC chemokine of Trachinotus ovatus, is involved in the antimicrobial immune response
TroCCL4, a CC chemokine of Trachinotus ovatus, is involved in the antimicrobial immune response
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TroCCL4 是卵形鲹鱼的 CC 趋化因子，参与抗菌免疫反应
DOI：
10.1016/j.fsi.2018.11.080
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发表时间：
2019-03
影响因子：
4.7
通讯作者：
Liu Chunsheng
中科院分区：
文献类型：
--
作者：
Sun Baiming;Lei Yang;Cao Zhenjie;Zhou Yongcan;Sun Yun;Wu Ying;Wang Shifeng;Guo Weiliang;Liu Chunsheng
CC趋化因子是在先天免疫系统中发挥重要作用的趋化因子的一个大的亚家族。迄今为止，已在鱼类中发现了几种CC趋化因子;然而，这些推定的趋化因子在硬骨鱼中的活性和功能仍然不清楚，尤其是在金鲷、卵形鲳鲹中。在这里，我们确定了CC趋化因子配体4从T。ovatus（TroCCL 4）的表达，并对其功能进行了研究。TroCCL 4含有294 bp的开放阅读框，其编码包含97个氨基酸的推定肽。TroCCL 4与人类和硬骨鱼中的其他CC趋化因子序列具有31.11%-78.35%的高氨基酸序列相似性，并且具有在其他CC趋化因子中保守的四个半胱氨酸残基。TroCCL 4与CC趋化因子中的巨噬细胞炎性蛋白（MIP）相关，在免疫器官中表达最丰富，在迟缓爱德华氏菌感染后呈时间依赖性显著上调。重组TroCCL 4（rTroCCL 4）诱导外周血白细胞迁移和头肾淋巴细胞增殖。此外，rTroCCL 4对大肠杆菌和大肠杆菌的生长也有抑制作用。tarda，表明抗微生物功能。体内分析结果表明，TroCCL 4过表达显著增强巨噬细胞的活化，上调白细胞介素1 β（IL-1β）、白细胞介素15（IL 15）、干扰素诱导的Mx蛋白（Mx）、肿瘤坏死因子α（TNFα）、补体C3和主要组织相容性复合物（MHC）Iα和IIα类基因的表达，并保护鱼组织免受细菌感染。相反，敲低TroCCL 4表达导致细菌在鱼组织中的传播和定殖增加。综上所述，我们的结果提供了证据表明TroCCL 4具有刺激白细胞和巨噬细胞并增强宿主免疫力以防御细菌感染的能力。
CC chemokines are a large subfamily of chemokines that play an important role in the innate immune system. To date, several CC chemokines have been identified in fish species; however, the activities and functions of these putative chemokines remain ambiguous in teleosts, especially in the golden pompano,Trachinotus ovatus. Here, we characterized CC chemokine ligand 4 fromT. ovatus(TroCCL4) and studied its functions. TroCCL4 contains a 294 bp open reading frame that encodes a putative peptide comprising 97 amino acids. TroCCL4 shares a high amino acid sequence similarity of 31.11%–78.35% with other CC chemokines sequences in humans and teleosts and has four cysteine residues that are conserved among other CC chemokines. TroCCL4 is also related to the macrophage inflammatory protein (MIP) group of CC chemokines.TroCCL4expression was most abundant in immune organs and significantly upregulated in a time-dependent manner following Edwardsiella tarda infection. Recombinant TroCCL4 (rTroCCL4) induced the migration of peripheral blood leukocytes and the cellular proliferation of head kidney lymphocytes. In addition, rTroCCL4 inhibited the growth ofEscherichia coliandE. tarda, indicating an antimicrobial function. Furthermore, the results ofin vivoanalysis showed thatTroCCL4overexpression inT. ovatussignificantly enhanced macrophage activation; upregulated the gene expression of interleukin 1-β (IL-1β), interleukin 15 (IL15), interferon-induced Mx protein (Mx), tumor necrosis factor α (TNFα), complement C3, and major histocompatibility complex (MHC) class Iα and class IIα; and protected against bacterial infection in fish tissues. In contrast, knockdown ofTroCCL4expression resulted in increased bacterial dissemination and colonization in fish tissues. Taken together, our results provide evidence indicating that TroCCL4 has the ability to stimulate leukocytes and macrophages and enhance host immunity to defend against bacterial infection.
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DOI：
10.1016/j.fsi.2016.10.012
发表时间：
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期刊：
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影响因子：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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